Tags

Type your tag names separated by a space and hit enter

IgA antiendomysial antibodies on the umbilical cord in diagnosing celiac disease. Sensitivity, specificity, and comparative evaluation with the traditional kit.
Scand J Gastroenterol 1996; 31(8):759-63SJ

Abstract

BACKGROUND

The possibility of assaying antiendomysial antibodies (EmA) on the human umbilical cord instead of monkey esophagus has recently been suggested. We therefore evaluated in patients with celiac disease (CD) the sensitivity and specificity of EmA and of antigliadin antibodies (AGA) for both umbilical cord and monkey esophagus.

METHODS

We studied 36 patients with CD and atrophy of the intestinal mucosa (median age, 1.4 years), 14 patients with CD on gluten-free diet for 8-12 months (median age, 3.0 years), 36 controls without gastrointestinal disease (median age, 4.0 years), and 72 patients with cow's milk protein enteropathy (CMPE) (median age, 1.2 years). AGA and EmA on monkey esophagus were assayed with commercially available kits; the slides with umbilical cord were prepared in our laboratory.

RESULTS

There was a perfect concordance between EmA results evaluated on umbilical cord and those on monkey esophagus; there was a doubtful result in only one case on human umbilical cord, which was positive with low titer on monkey esophagus. EmA specificity was 100%; the specificity of AGA IgG varied between 72% and 94% and of AGA IgA between 90% and 100% depending on whether controls without gastrointestinal disorders or patients with CMPE were considered. EmA sensitivity was 97%, AGA IgG was 89%, and AGA IgA 72% sensitive. The only false negative for EmA was positive for AGA IgG and AGA IgA.

CONCLUSIONS

Using human umbilical cord as a substrate for EmA may provide the same sensitivity and specificity as offered by the test using monkey esophagus substrate, thus reducing costs and avoiding the use of endangered species.

Authors+Show Affiliations

Dept. of Internal Medicine, University of Palermo, Italy.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8858743

Citation

Carroccio, A, et al. "IgA Antiendomysial Antibodies On the Umbilical Cord in Diagnosing Celiac Disease. Sensitivity, Specificity, and Comparative Evaluation With the Traditional Kit." Scandinavian Journal of Gastroenterology, vol. 31, no. 8, 1996, pp. 759-63.
Carroccio A, Cavataio F, Iacono G, et al. IgA antiendomysial antibodies on the umbilical cord in diagnosing celiac disease. Sensitivity, specificity, and comparative evaluation with the traditional kit. Scand J Gastroenterol. 1996;31(8):759-63.
Carroccio, A., Cavataio, F., Iacono, G., Agate, V., Ippolito, S., Kazmierska, I., ... Montalto, G. (1996). IgA antiendomysial antibodies on the umbilical cord in diagnosing celiac disease. Sensitivity, specificity, and comparative evaluation with the traditional kit. Scandinavian Journal of Gastroenterology, 31(8), pp. 759-63.
Carroccio A, et al. IgA Antiendomysial Antibodies On the Umbilical Cord in Diagnosing Celiac Disease. Sensitivity, Specificity, and Comparative Evaluation With the Traditional Kit. Scand J Gastroenterol. 1996;31(8):759-63. PubMed PMID: 8858743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgA antiendomysial antibodies on the umbilical cord in diagnosing celiac disease. Sensitivity, specificity, and comparative evaluation with the traditional kit. AU - Carroccio,A, AU - Cavataio,F, AU - Iacono,G, AU - Agate,V, AU - Ippolito,S, AU - Kazmierska,I, AU - Campagna,P, AU - Soresi,M, AU - Montalto,G, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 759 EP - 63 JF - Scandinavian journal of gastroenterology JO - Scand. J. Gastroenterol. VL - 31 IS - 8 N2 - BACKGROUND: The possibility of assaying antiendomysial antibodies (EmA) on the human umbilical cord instead of monkey esophagus has recently been suggested. We therefore evaluated in patients with celiac disease (CD) the sensitivity and specificity of EmA and of antigliadin antibodies (AGA) for both umbilical cord and monkey esophagus. METHODS: We studied 36 patients with CD and atrophy of the intestinal mucosa (median age, 1.4 years), 14 patients with CD on gluten-free diet for 8-12 months (median age, 3.0 years), 36 controls without gastrointestinal disease (median age, 4.0 years), and 72 patients with cow's milk protein enteropathy (CMPE) (median age, 1.2 years). AGA and EmA on monkey esophagus were assayed with commercially available kits; the slides with umbilical cord were prepared in our laboratory. RESULTS: There was a perfect concordance between EmA results evaluated on umbilical cord and those on monkey esophagus; there was a doubtful result in only one case on human umbilical cord, which was positive with low titer on monkey esophagus. EmA specificity was 100%; the specificity of AGA IgG varied between 72% and 94% and of AGA IgA between 90% and 100% depending on whether controls without gastrointestinal disorders or patients with CMPE were considered. EmA sensitivity was 97%, AGA IgG was 89%, and AGA IgA 72% sensitive. The only false negative for EmA was positive for AGA IgG and AGA IgA. CONCLUSIONS: Using human umbilical cord as a substrate for EmA may provide the same sensitivity and specificity as offered by the test using monkey esophagus substrate, thus reducing costs and avoiding the use of endangered species. SN - 0036-5521 UR - https://www.unboundmedicine.com/medline/citation/8858743/IgA_antiendomysial_antibodies_on_the_umbilical_cord_in_diagnosing_celiac_disease__Sensitivity_specificity_and_comparative_evaluation_with_the_traditional_kit_ L2 - http://www.tandfonline.com/doi/full/10.3109/00365529609010348 DB - PRIME DP - Unbound Medicine ER -