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A 700 bp cis-acting region controls mating-type dependent recombination along the entire left arm of yeast chromosome III.
Cell. 1996 Oct 18; 87(2):277-85.Cell

Abstract

Homothallic switching of the mating-type MATa gene in Saccharomyces cerevisiae results from replacement by gene conversion of MAT-Ya DNA with Y(alpha) sequences copied from one of two unexpressed donors. MATa preferentially recombines with HML(alpha), located near the left end of chromosome III, but can use HMR(alpha), near the right chromosome end. MATa donor preference depends on a 700 bp orientation-independent cis-acting recombination enhancer, located 17 kb proximal to HML. Deletion of this element markedly reduces MATa's use of a donor inserted at any of four different locations along the leftmost 92 kb of chromosome III. This enhancer is sufficient for donor activation, since it stimulates use of the "wrong" donor, when it is inserted 7 kb proximal to HMR.

Authors+Show Affiliations

Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8861911

Citation

Wu, X, and J E. Haber. "A 700 Bp Cis-acting Region Controls Mating-type Dependent Recombination Along the Entire Left Arm of Yeast Chromosome III." Cell, vol. 87, no. 2, 1996, pp. 277-85.
Wu X, Haber JE. A 700 bp cis-acting region controls mating-type dependent recombination along the entire left arm of yeast chromosome III. Cell. 1996;87(2):277-85.
Wu, X., & Haber, J. E. (1996). A 700 bp cis-acting region controls mating-type dependent recombination along the entire left arm of yeast chromosome III. Cell, 87(2), 277-85.
Wu X, Haber JE. A 700 Bp Cis-acting Region Controls Mating-type Dependent Recombination Along the Entire Left Arm of Yeast Chromosome III. Cell. 1996 Oct 18;87(2):277-85. PubMed PMID: 8861911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A 700 bp cis-acting region controls mating-type dependent recombination along the entire left arm of yeast chromosome III. AU - Wu,X, AU - Haber,J E, PY - 1996/10/18/pubmed PY - 1996/10/18/medline PY - 1996/10/18/entrez SP - 277 EP - 85 JF - Cell JO - Cell VL - 87 IS - 2 N2 - Homothallic switching of the mating-type MATa gene in Saccharomyces cerevisiae results from replacement by gene conversion of MAT-Ya DNA with Y(alpha) sequences copied from one of two unexpressed donors. MATa preferentially recombines with HML(alpha), located near the left end of chromosome III, but can use HMR(alpha), near the right chromosome end. MATa donor preference depends on a 700 bp orientation-independent cis-acting recombination enhancer, located 17 kb proximal to HML. Deletion of this element markedly reduces MATa's use of a donor inserted at any of four different locations along the leftmost 92 kb of chromosome III. This enhancer is sufficient for donor activation, since it stimulates use of the "wrong" donor, when it is inserted 7 kb proximal to HMR. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/8861911/A_700_bp_cis_acting_region_controls_mating_type_dependent_recombination_along_the_entire_left_arm_of_yeast_chromosome_III_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(00)81345-8 DB - PRIME DP - Unbound Medicine ER -