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PAF and hematopoiesis. VIII. Biosynthesis and metabolism of PAF by human bone marrow stromal cells.
Exp Hematol. 1996 Sep; 24(11):1327-32.EH

Abstract

Human bone marrow stromal cells were studied for their ability to synthesize and to metabolize platelet-activating factor (PAF), a lipidic compound with potent immunoregulatory properties. When stimulated with 2 microM calcium ionophore for 60 minutes, cultures of stromal cells increased their PAF production (3.52 +/- 0.91 ng/1 x 10(6) cells) compared with controls (0.82 +/- 0.13 ng/1 x 10(6) cells). Addition of exogenous lyso PAF (100 nM) and acetyl-CoA (100 microM) during calcium ionophore stimulation did not change the PAF production. The synthesis of PAF was not influenced by the concentration of albumin in the incubation buffer. The PAF from stromal cells exhibited a hexadecyl chain at the sn-1 position of the molecule, as determined by reverse-phase HPLC. While stromal cells contained low amounts of PAF acetylhydrolase activity and did not secrete it in the culture medium, they metabolized exogenous PAF with 1-alkyl-2-acyl-glycero-phosphocholine and neutral lipids as the major metabolic products. The present results are the first to demonstrate the synthesis and metabolism of PAF by human bone marrow stromal cells. These data suggest that they might be a source of the PAF found in the human bone marrow and/or might be important in the regulation of its levels. The role of PAF on the proliferation and functions of human hematopoietic cells deserves investigation.

Authors+Show Affiliations

Denizot Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, CHRU Dupuytren, Limoges, France.
Rougier F
No affiliation info available
Dupuis F
No affiliation info available
Verger C
No affiliation info available
Dulery C
No affiliation info available
Laskar M
No affiliation info available
Aldigier JC
No affiliation info available
Praloran V
No affiliation info available

MeSH

Bone MarrowBone Marrow CellsCells, CulturedHematopoiesisHumansPlatelet Activating FactorStromal Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8862444

Citation

Denizot, Y, et al. "PAF and Hematopoiesis. VIII. Biosynthesis and Metabolism of PAF By Human Bone Marrow Stromal Cells." Experimental Hematology, vol. 24, no. 11, 1996, pp. 1327-32.
Denizot Y, Rougier F, Dupuis F, et al. PAF and hematopoiesis. VIII. Biosynthesis and metabolism of PAF by human bone marrow stromal cells. Exp Hematol. 1996;24(11):1327-32.
Denizot, Y., Rougier, F., Dupuis, F., Verger, C., Dulery, C., Laskar, M., Aldigier, J. C., & Praloran, V. (1996). PAF and hematopoiesis. VIII. Biosynthesis and metabolism of PAF by human bone marrow stromal cells. Experimental Hematology, 24(11), 1327-32.
Denizot Y, et al. PAF and Hematopoiesis. VIII. Biosynthesis and Metabolism of PAF By Human Bone Marrow Stromal Cells. Exp Hematol. 1996;24(11):1327-32. PubMed PMID: 8862444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PAF and hematopoiesis. VIII. Biosynthesis and metabolism of PAF by human bone marrow stromal cells. AU - Denizot,Y, AU - Rougier,F, AU - Dupuis,F, AU - Verger,C, AU - Dulery,C, AU - Laskar,M, AU - Aldigier,J C, AU - Praloran,V, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - 1327 EP - 32 JF - Experimental hematology JO - Exp Hematol VL - 24 IS - 11 N2 - Human bone marrow stromal cells were studied for their ability to synthesize and to metabolize platelet-activating factor (PAF), a lipidic compound with potent immunoregulatory properties. When stimulated with 2 microM calcium ionophore for 60 minutes, cultures of stromal cells increased their PAF production (3.52 +/- 0.91 ng/1 x 10(6) cells) compared with controls (0.82 +/- 0.13 ng/1 x 10(6) cells). Addition of exogenous lyso PAF (100 nM) and acetyl-CoA (100 microM) during calcium ionophore stimulation did not change the PAF production. The synthesis of PAF was not influenced by the concentration of albumin in the incubation buffer. The PAF from stromal cells exhibited a hexadecyl chain at the sn-1 position of the molecule, as determined by reverse-phase HPLC. While stromal cells contained low amounts of PAF acetylhydrolase activity and did not secrete it in the culture medium, they metabolized exogenous PAF with 1-alkyl-2-acyl-glycero-phosphocholine and neutral lipids as the major metabolic products. The present results are the first to demonstrate the synthesis and metabolism of PAF by human bone marrow stromal cells. These data suggest that they might be a source of the PAF found in the human bone marrow and/or might be important in the regulation of its levels. The role of PAF on the proliferation and functions of human hematopoietic cells deserves investigation. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/8862444/PAF_and_hematopoiesis__VIII__Biosynthesis_and_metabolism_of_PAF_by_human_bone_marrow_stromal_cells_ DB - PRIME DP - Unbound Medicine ER -