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Yeast thiol-dependent protector protein expression enhances the resistance of Escherichia coli to hydrogen peroxide.
Biochem Mol Biol Int. 1996 Aug; 39(5):1007-15.BM

Abstract

A soluble protein from Saccharomyces cerevisiae specifically provides protection against a thiol-containing oxidation system but not against an oxidation system without thiol. This 25-kDa protein was thus named thiol-dependent protector protein (TPP). The role of TPP in the cellular defense against oxidative stress was investigated in Escherichia coli containing an expression vector with a yeast genomic DNA fragment that encodes TPP (strain YP) and mutants in which the catalytically essential amino acid cysteine (Cys-47) has been replaced with alanine (strain YPC47A) or tryptophan (Trp-82) has been replaced with phenylalanine (strain YPW82F) by a site directed mutagenesis. There was a distinct difference between these three strains in regards to growth inhibition kinetics, viability, modulation of activities of superoxide dismutase and catalase, and the accumulation of oxidized proteins. These results suggest that TPP may play a direct role in the cellular defense against oxidative stress by functioning as an antioxidant protein.

Authors+Show Affiliations

Ahn SM
Department of Biochemistry, Kyungpook National University, Taegu, Korea.
Lee SM
No affiliation info available
Chung T
No affiliation info available
Kim K
No affiliation info available
Park JW
No affiliation info available

MeSH

AlanineAnimalsBinding SitesBlotting, WesternCatalaseCell DeathCell DivisionCysteineDNA, ComplementaryDose-Response Relationship, DrugEscherichia coliFungal ProteinsGene Expression Regulation, BacterialHydrogen PeroxideIsopropyl ThiogalactosideMutagenesis, Site-DirectedNeoplasm ProteinsOxidantsOxidation-ReductionOxidative StressPeroxidasesPeroxiredoxinsProteinsRabbitsRecombinant ProteinsSaccharomyces cerevisiaeSuperoxide Dismutase

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8866018

Citation

Ahn, S M., et al. "Yeast Thiol-dependent Protector Protein Expression Enhances the Resistance of Escherichia Coli to Hydrogen Peroxide." Biochemistry and Molecular Biology International, vol. 39, no. 5, 1996, pp. 1007-15.
Ahn SM, Lee SM, Chung T, et al. Yeast thiol-dependent protector protein expression enhances the resistance of Escherichia coli to hydrogen peroxide. Biochem Mol Biol Int. 1996;39(5):1007-15.
Ahn, S. M., Lee, S. M., Chung, T., Kim, K., & Park, J. W. (1996). Yeast thiol-dependent protector protein expression enhances the resistance of Escherichia coli to hydrogen peroxide. Biochemistry and Molecular Biology International, 39(5), 1007-15.
Ahn SM, et al. Yeast Thiol-dependent Protector Protein Expression Enhances the Resistance of Escherichia Coli to Hydrogen Peroxide. Biochem Mol Biol Int. 1996;39(5):1007-15. PubMed PMID: 8866018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Yeast thiol-dependent protector protein expression enhances the resistance of Escherichia coli to hydrogen peroxide. AU - Ahn,S M, AU - Lee,S M, AU - Chung,T, AU - Kim,K, AU - Park,J W, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 1007 EP - 15 JF - Biochemistry and molecular biology international JO - Biochem Mol Biol Int VL - 39 IS - 5 N2 - A soluble protein from Saccharomyces cerevisiae specifically provides protection against a thiol-containing oxidation system but not against an oxidation system without thiol. This 25-kDa protein was thus named thiol-dependent protector protein (TPP). The role of TPP in the cellular defense against oxidative stress was investigated in Escherichia coli containing an expression vector with a yeast genomic DNA fragment that encodes TPP (strain YP) and mutants in which the catalytically essential amino acid cysteine (Cys-47) has been replaced with alanine (strain YPC47A) or tryptophan (Trp-82) has been replaced with phenylalanine (strain YPW82F) by a site directed mutagenesis. There was a distinct difference between these three strains in regards to growth inhibition kinetics, viability, modulation of activities of superoxide dismutase and catalase, and the accumulation of oxidized proteins. These results suggest that TPP may play a direct role in the cellular defense against oxidative stress by functioning as an antioxidant protein. SN - 1039-9712 UR - https://www.unboundmedicine.com/medline/citation/8866018/Yeast_thiol_dependent_protector_protein_expression_enhances_the_resistance_of_Escherichia_coli_to_hydrogen_peroxide_ DB - PRIME DP - Unbound Medicine ER -