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Growth characteristics of bifidobacteria in infant formulas.
J Dairy Sci. 1996 Jul; 79(7):1146-55.JD

Abstract

Four species of bifidobacteria, Bifidobacterium bifidum (ATCC 15696), Bifidobacterium breve (ATCC 15700), Bifidobacterium infantis (ATCC 15697), and Bifidobacterium longum (ATCC 15708), were grown anaerobically at 37 degrees C in lactobacilli MRS broth with 0.5% cysteine-HC1 and inoculated at 2.5% into three types of infant formula (based on soy, or milk, or casein hydrolysate) and in nonfat milk followed by incubation at 37 degrees C for 24 h. In most cases, the logarithmic phase of growth for all species varied from the first 8 to 12 h postinoculation. Generation times for B. longum and B. breve were similar, and times for B. infantis were shortest, in all of the formulas. Trends for lactic acid production for all species in all the formulas were similar to trends for acetic acid production. Counts for formulas based on soy or milk were similar for all species except B. bifidum, and those for casein-hydrolyzed formula were always lowest for all species except B. bifidum, for which count was maximal with the formula based on soy. Results suggest that growth characteristics of bifidobacteria in infant formula were species specific and formula dependent and that growth was maximal in the formula based on milk.

Authors+Show Affiliations

Minnesota-South Dakota Dairy Foods Research Center, Dairy Science Department, South Dakota State University, Brookings 57007-0647, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8872711

Citation

Dubey, U K., and V V. Mistry. "Growth Characteristics of Bifidobacteria in Infant Formulas." Journal of Dairy Science, vol. 79, no. 7, 1996, pp. 1146-55.
Dubey UK, Mistry VV. Growth characteristics of bifidobacteria in infant formulas. J Dairy Sci. 1996;79(7):1146-55.
Dubey, U. K., & Mistry, V. V. (1996). Growth characteristics of bifidobacteria in infant formulas. Journal of Dairy Science, 79(7), 1146-55.
Dubey UK, Mistry VV. Growth Characteristics of Bifidobacteria in Infant Formulas. J Dairy Sci. 1996;79(7):1146-55. PubMed PMID: 8872711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth characteristics of bifidobacteria in infant formulas. AU - Dubey,U K, AU - Mistry,V V, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 1146 EP - 55 JF - Journal of dairy science JO - J Dairy Sci VL - 79 IS - 7 N2 - Four species of bifidobacteria, Bifidobacterium bifidum (ATCC 15696), Bifidobacterium breve (ATCC 15700), Bifidobacterium infantis (ATCC 15697), and Bifidobacterium longum (ATCC 15708), were grown anaerobically at 37 degrees C in lactobacilli MRS broth with 0.5% cysteine-HC1 and inoculated at 2.5% into three types of infant formula (based on soy, or milk, or casein hydrolysate) and in nonfat milk followed by incubation at 37 degrees C for 24 h. In most cases, the logarithmic phase of growth for all species varied from the first 8 to 12 h postinoculation. Generation times for B. longum and B. breve were similar, and times for B. infantis were shortest, in all of the formulas. Trends for lactic acid production for all species in all the formulas were similar to trends for acetic acid production. Counts for formulas based on soy or milk were similar for all species except B. bifidum, and those for casein-hydrolyzed formula were always lowest for all species except B. bifidum, for which count was maximal with the formula based on soy. Results suggest that growth characteristics of bifidobacteria in infant formula were species specific and formula dependent and that growth was maximal in the formula based on milk. SN - 0022-0302 UR - https://www.unboundmedicine.com/medline/citation/8872711/Growth_characteristics_of_bifidobacteria_in_infant_formulas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(96)76468-8 DB - PRIME DP - Unbound Medicine ER -