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Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography.
Electrophoresis. 1996 Aug; 17(8):1357-60.E

Abstract

Amphoteric poly(Lys-[Glu1.0-DL-Ala4.1]), (EAK) and anionic poly(Lys-Ac-Glu0.98-DL-Ala3.98]), (AcEAK) branched chain polypeptides were analyzed by capillary electrophoresis (CE) and micellar elektrokinetic chromatography (MEKC) in the following buffers. A1: 0.25 N triethyl ammonium phosphate (TEAP) buffer (pH 2.25); A2: 100 mM sodium dodecyl sulfate (SDS) in buffer A1; B1: Na-borate buffer (pH 7.7); B2: 100 mM SDS in buffer B1; C1: Na-borate buffer (pH 11.0); C2: 100 mM SDS in buffer C1. Both EAK and AcEAK could be separated by a CE mechanism at pH 2.25 and by an MEKC mechanism at pH 11.0. Optimum results were achieved with CE in buffer A1 and with MEKC in buffer C2.

Authors+Show Affiliations

Idei M
Semmelweis University of Medicine, Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Budapest, Hungary. miki@puskin.sote.hu
Dibó G
No affiliation info available
Bogdán K
No affiliation info available
Mezö G
No affiliation info available
Horváth A
No affiliation info available
Erchegyi J
No affiliation info available
Mészáros G
No affiliation info available
Teplán I
No affiliation info available
Kéri G
No affiliation info available
Hudecz F
No affiliation info available

MeSH

BuffersChromatography, LiquidElectrophoresis, CapillaryHydrogen-Ion ConcentrationMicellesPeptides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8874062

Citation

Idei, M, et al. "Analysis of Macromolecular Branched Chain Polypeptides By Capillary Electrophoresis and Micellar Electrokinetic Chromatography." Electrophoresis, vol. 17, no. 8, 1996, pp. 1357-60.
Idei M, Dibó G, Bogdán K, et al. Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography. Electrophoresis. 1996;17(8):1357-60.
Idei, M., Dibó, G., Bogdán, K., Mezö, G., Horváth, A., Erchegyi, J., Mészáros, G., Teplán, I., Kéri, G., & Hudecz, F. (1996). Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography. Electrophoresis, 17(8), 1357-60.
Idei M, et al. Analysis of Macromolecular Branched Chain Polypeptides By Capillary Electrophoresis and Micellar Electrokinetic Chromatography. Electrophoresis. 1996;17(8):1357-60. PubMed PMID: 8874062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography. AU - Idei,M, AU - Dibó,G, AU - Bogdán,K, AU - Mezö,G, AU - Horváth,A, AU - Erchegyi,J, AU - Mészáros,G, AU - Teplán,I, AU - Kéri,G, AU - Hudecz,F, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 1357 EP - 60 JF - Electrophoresis JO - Electrophoresis VL - 17 IS - 8 N2 - Amphoteric poly(Lys-[Glu1.0-DL-Ala4.1]), (EAK) and anionic poly(Lys-Ac-Glu0.98-DL-Ala3.98]), (AcEAK) branched chain polypeptides were analyzed by capillary electrophoresis (CE) and micellar elektrokinetic chromatography (MEKC) in the following buffers. A1: 0.25 N triethyl ammonium phosphate (TEAP) buffer (pH 2.25); A2: 100 mM sodium dodecyl sulfate (SDS) in buffer A1; B1: Na-borate buffer (pH 7.7); B2: 100 mM SDS in buffer B1; C1: Na-borate buffer (pH 11.0); C2: 100 mM SDS in buffer C1. Both EAK and AcEAK could be separated by a CE mechanism at pH 2.25 and by an MEKC mechanism at pH 11.0. Optimum results were achieved with CE in buffer A1 and with MEKC in buffer C2. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/8874062/Analysis_of_macromolecular_branched_chain_polypeptides_by_capillary_electrophoresis_and_micellar_electrokinetic_chromatography_ L2 - https://doi.org/10.1002/elps.1150170813 DB - PRIME DP - Unbound Medicine ER -