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Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control?
Mol Gen Genet. 1996 Sep 25; 252(4):470-82.MG

Abstract

Saccharomyces cerevisiae cells show under suboptimal growth conditions a complex response that leads to the acquisition of tolerance to different types of environmental stress. This response is characterised by enhanced expression of a number of genes which contain so-called stress-responsive elements (STREs) in their promoters. In addition, the cells accumulate under suboptimal conditions the putative stress protectant trehalose. In this work, we have examined the expression of four genes encoding subunits of the trehalose synthase complex, GGS1/TPS1, TPS2, TPS3 and TSL1. We show that expression of these genes is coregulated under stress conditions. Like for many other genes containing STREs, expression of the trehalose synthase genes is also induced by heat and osmotic stress and by nutrient starvation, and negatively regulated by the Ras-cAMP pathway. However, during fermentative growth only TSL1 shows an expression pattern like that of the STRE-controlled genes CTT1 and SSA3, while expression of the three other trehalose synthase genes is only transiently down-regulated. This difference in expression might be related to the known requirement of trehalose biosynthesis for the control of yeast glycolysis and hence for fermentative growth. We conclude that the mere presence in the promoter of (an) active STRE(s) does not necessarily imply complete coregulation of expression. Additional mechanisms appear to fine tune the activity of STREs in order to adapt the expression of the downstream genes to specific requirements.

Authors+Show Affiliations

Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Leuven-Heverlee, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8879249

Citation

Winderickx, J, et al. "Regulation of Genes Encoding Subunits of the Trehalose Synthase Complex in Saccharomyces Cerevisiae: Novel Variations of STRE-mediated Transcription Control?" Molecular & General Genetics : MGG, vol. 252, no. 4, 1996, pp. 470-82.
Winderickx J, de Winde JH, Crauwels M, et al. Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control? Mol Gen Genet. 1996;252(4):470-82.
Winderickx, J., de Winde, J. H., Crauwels, M., Hino, A., Hohmann, S., Van Dijck, P., & Thevelein, J. M. (1996). Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control? Molecular & General Genetics : MGG, 252(4), 470-82.
Winderickx J, et al. Regulation of Genes Encoding Subunits of the Trehalose Synthase Complex in Saccharomyces Cerevisiae: Novel Variations of STRE-mediated Transcription Control. Mol Gen Genet. 1996 Sep 25;252(4):470-82. PubMed PMID: 8879249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of genes encoding subunits of the trehalose synthase complex in Saccharomyces cerevisiae: novel variations of STRE-mediated transcription control? AU - Winderickx,J, AU - de Winde,J H, AU - Crauwels,M, AU - Hino,A, AU - Hohmann,S, AU - Van Dijck,P, AU - Thevelein,J M, PY - 1996/9/25/pubmed PY - 1996/9/25/medline PY - 1996/9/25/entrez SP - 470 EP - 82 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 252 IS - 4 N2 - Saccharomyces cerevisiae cells show under suboptimal growth conditions a complex response that leads to the acquisition of tolerance to different types of environmental stress. This response is characterised by enhanced expression of a number of genes which contain so-called stress-responsive elements (STREs) in their promoters. In addition, the cells accumulate under suboptimal conditions the putative stress protectant trehalose. In this work, we have examined the expression of four genes encoding subunits of the trehalose synthase complex, GGS1/TPS1, TPS2, TPS3 and TSL1. We show that expression of these genes is coregulated under stress conditions. Like for many other genes containing STREs, expression of the trehalose synthase genes is also induced by heat and osmotic stress and by nutrient starvation, and negatively regulated by the Ras-cAMP pathway. However, during fermentative growth only TSL1 shows an expression pattern like that of the STRE-controlled genes CTT1 and SSA3, while expression of the three other trehalose synthase genes is only transiently down-regulated. This difference in expression might be related to the known requirement of trehalose biosynthesis for the control of yeast glycolysis and hence for fermentative growth. We conclude that the mere presence in the promoter of (an) active STRE(s) does not necessarily imply complete coregulation of expression. Additional mechanisms appear to fine tune the activity of STREs in order to adapt the expression of the downstream genes to specific requirements. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/8879249/Regulation_of_genes_encoding_subunits_of_the_trehalose_synthase_complex_in_Saccharomyces_cerevisiae:_novel_variations_of_STRE_mediated_transcription_control L2 - http://www.yeastgenome.org/reference/8879249 DB - PRIME DP - Unbound Medicine ER -