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Clonal analysis by chromosome 11 microsatellite-PCR of microdissected parathyroid tumors from MEN 1 patients.
Biochem Biophys Res Commun. 1996 Oct 23; 227(3):736-42.BB

Abstract

Loss of heterozygosity (LOH) at chromosome 11q13 loci has been described in the majority of larger parathyroid tumors from patients affected by Multiple Endocrine Neoplasia type 1 (MEN 1) syndrome. Since classical histology of the whole parathyroid gland does not permit a clear morpho-genetic correlation, the clonal composition of abnormal parathyroid tissue was analyzed in DNA obtained from single nodules and non-nodular areas within MEN 1 parathyroid lesions. Microdissected sections were analyzed by chromosome 11q13 microsatellite-PCR for LOH and by patterns of X-inactivation. We detected LOH for chromosome 11q13 loci in at least one microdissected area for each familial MEN 1 patient, but not in the single sporadic case. X-inactivation pattern of two "clonal" tumors exhibited a polyclonal cell composition of these microdissected samples, indicating the existence of a genetic heterogeneity in MEN 1 parathyroid microareas exhibiting a "clonal" pattern for allelic losses.

Authors+Show Affiliations

Department of Clinical Physiopathology, University of Florence, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8886003

Citation

Morelli, A, et al. "Clonal Analysis By Chromosome 11 microsatellite-PCR of Microdissected Parathyroid Tumors From MEN 1 Patients." Biochemical and Biophysical Research Communications, vol. 227, no. 3, 1996, pp. 736-42.
Morelli A, Falchetti A, Amorosi A, et al. Clonal analysis by chromosome 11 microsatellite-PCR of microdissected parathyroid tumors from MEN 1 patients. Biochem Biophys Res Commun. 1996;227(3):736-42.
Morelli, A., Falchetti, A., Amorosi, A., Tonelli, F., Bearzi, I., Ranaldi, R., Tomassetti, P., & Brandi, M. L. (1996). Clonal analysis by chromosome 11 microsatellite-PCR of microdissected parathyroid tumors from MEN 1 patients. Biochemical and Biophysical Research Communications, 227(3), 736-42.
Morelli A, et al. Clonal Analysis By Chromosome 11 microsatellite-PCR of Microdissected Parathyroid Tumors From MEN 1 Patients. Biochem Biophys Res Commun. 1996 Oct 23;227(3):736-42. PubMed PMID: 8886003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clonal analysis by chromosome 11 microsatellite-PCR of microdissected parathyroid tumors from MEN 1 patients. AU - Morelli,A, AU - Falchetti,A, AU - Amorosi,A, AU - Tonelli,F, AU - Bearzi,I, AU - Ranaldi,R, AU - Tomassetti,P, AU - Brandi,M L, PY - 1996/10/23/pubmed PY - 2000/3/29/medline PY - 1996/10/23/entrez SP - 736 EP - 42 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 227 IS - 3 N2 - Loss of heterozygosity (LOH) at chromosome 11q13 loci has been described in the majority of larger parathyroid tumors from patients affected by Multiple Endocrine Neoplasia type 1 (MEN 1) syndrome. Since classical histology of the whole parathyroid gland does not permit a clear morpho-genetic correlation, the clonal composition of abnormal parathyroid tissue was analyzed in DNA obtained from single nodules and non-nodular areas within MEN 1 parathyroid lesions. Microdissected sections were analyzed by chromosome 11q13 microsatellite-PCR for LOH and by patterns of X-inactivation. We detected LOH for chromosome 11q13 loci in at least one microdissected area for each familial MEN 1 patient, but not in the single sporadic case. X-inactivation pattern of two "clonal" tumors exhibited a polyclonal cell composition of these microdissected samples, indicating the existence of a genetic heterogeneity in MEN 1 parathyroid microareas exhibiting a "clonal" pattern for allelic losses. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/8886003/Clonal_analysis_by_chromosome_11_microsatellite_PCR_of_microdissected_parathyroid_tumors_from_MEN_1_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(96)91578-8 DB - PRIME DP - Unbound Medicine ER -