Tags

Type your tag names separated by a space and hit enter

Zinc deficiency and the desaturation of linoleic acid in rats force-fed fat-free diets.
Biol Trace Elem Res. 1996 Aug; 54(2):173-83.BT

Abstract

Recent studies with rats force-fed zinc-deficient diets containing various types of fat failed to demonstrate a role of zinc in desaturation of linoleic acid. The present study was conducted to investigate the effect of zinc deficiency on desaturation of linoleic acid in rats that were initially force-fed fat-free diets to stimulate activity of desaturases. Therefore, rats were fed zinc-adequate and zinc-deficient fat-free diets for 6 d. After that period, the groups were divided and half of the rats continued feeding the fat-free diet for another 3.5 d whereas the other half was switched to a fat diet by supplementing the fat-free diet with 5% safflower oil. In order to assess desaturation of linoleic acid, fatty acid compositions of liver phosphatidylcholine, -ethanolamine, and -serine were considered, particularly levels of individual (n-6) polyunsaturated fatty acids (PUFA). Levels of total and individual (n-6) PUFA were similar in zinc-adequate and zinc-deficient rats fed the fat-free diet throughout the experiment. Addition of 5% safflower oil increased levels of total and individual (n-6) PUFA in both zinc-adequate and zinc-deficient rats. However, total (n-6) PUFA in all types of phospholipids were higher in zinc-adequate rats than in zinc-deficient rats. Additionally, in zinc-deficient rats there were changes of (n-6) PUFA levels typical for impaired delta 5 and delta 6 desaturation: linoleic acid and dihomo-gamma-linolenic acid were elevated; arachidonic acid, docosatetraenoic acid, and docosapentaenoic were lowered by zinc deficiency. Therefore, the study shows that zinc deficiency impairs desaturation of linoleic acid in rats force-fed fat-free diets and therefore supports results from former convential zinc deficiency experiments suggesting a role of zinc for desaturation of linoleic acid.

Authors+Show Affiliations

Institut für Ernährungsphysiologie der Technischen, Universität München, Freising, Germany.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8886317

Citation

Eder, K, and M Kirchgessner. "Zinc Deficiency and the Desaturation of Linoleic Acid in Rats Force-fed Fat-free Diets." Biological Trace Element Research, vol. 54, no. 2, 1996, pp. 173-83.
Eder K, Kirchgessner M. Zinc deficiency and the desaturation of linoleic acid in rats force-fed fat-free diets. Biol Trace Elem Res. 1996;54(2):173-83.
Eder, K., & Kirchgessner, M. (1996). Zinc deficiency and the desaturation of linoleic acid in rats force-fed fat-free diets. Biological Trace Element Research, 54(2), 173-83.
Eder K, Kirchgessner M. Zinc Deficiency and the Desaturation of Linoleic Acid in Rats Force-fed Fat-free Diets. Biol Trace Elem Res. 1996;54(2):173-83. PubMed PMID: 8886317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zinc deficiency and the desaturation of linoleic acid in rats force-fed fat-free diets. AU - Eder,K, AU - Kirchgessner,M, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 173 EP - 83 JF - Biological trace element research JO - Biol Trace Elem Res VL - 54 IS - 2 N2 - Recent studies with rats force-fed zinc-deficient diets containing various types of fat failed to demonstrate a role of zinc in desaturation of linoleic acid. The present study was conducted to investigate the effect of zinc deficiency on desaturation of linoleic acid in rats that were initially force-fed fat-free diets to stimulate activity of desaturases. Therefore, rats were fed zinc-adequate and zinc-deficient fat-free diets for 6 d. After that period, the groups were divided and half of the rats continued feeding the fat-free diet for another 3.5 d whereas the other half was switched to a fat diet by supplementing the fat-free diet with 5% safflower oil. In order to assess desaturation of linoleic acid, fatty acid compositions of liver phosphatidylcholine, -ethanolamine, and -serine were considered, particularly levels of individual (n-6) polyunsaturated fatty acids (PUFA). Levels of total and individual (n-6) PUFA were similar in zinc-adequate and zinc-deficient rats fed the fat-free diet throughout the experiment. Addition of 5% safflower oil increased levels of total and individual (n-6) PUFA in both zinc-adequate and zinc-deficient rats. However, total (n-6) PUFA in all types of phospholipids were higher in zinc-adequate rats than in zinc-deficient rats. Additionally, in zinc-deficient rats there were changes of (n-6) PUFA levels typical for impaired delta 5 and delta 6 desaturation: linoleic acid and dihomo-gamma-linolenic acid were elevated; arachidonic acid, docosatetraenoic acid, and docosapentaenoic were lowered by zinc deficiency. Therefore, the study shows that zinc deficiency impairs desaturation of linoleic acid in rats force-fed fat-free diets and therefore supports results from former convential zinc deficiency experiments suggesting a role of zinc for desaturation of linoleic acid. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/8886317/Zinc_deficiency_and_the_desaturation_of_linoleic_acid_in_rats_force_fed_fat_free_diets_ L2 - https://dx.doi.org/10.1007/BF02786264 DB - PRIME DP - Unbound Medicine ER -