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Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study.
Anat Rec. 1996 Oct; 246(2):185-94.AR

Abstract

BACKGROUND

The aim of this study was to characterize the myosin heavy chain (MyHC) isoforms present in equine skeletal muscle.

METHODS

Muscle biopsies were removed from the superficial region of the gluteus medius muscle of five mature horses and analyzed by immunohistochemistry (using a battery of monoclonal antibodies specific for rat MyHC isoforms) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

RESULTS

Immunohistochemistry allowed subdivision of three different muscle fiber populations containing a single MyHC, one slow and two fast, and two hybrid populations, one containing slow and fast MyHCs and another with both fast-MyHC isoforms. Electrophoresis of MyHC confirmed the existence of three resolvable bands, with an electrophoretic mobility parallel to type I, IIa, and IIx rat MyHCs. The identities of two of these MyHCs were easily comparable with slow type I and fast type IIa MyHCs from rat skeletal muscle. However, a precise identification of the second fast MyHC was not made.

CONCLUSIONS

These results show the presence of three different MyHC isoforms in mature equine skeletal muscle, whose differential distribution defines three fiber types containing a single MyHC and two hybrid fiber populations containing either both slow and fast type IIa MyHCs or both fast MyHC isoforms.

Authors+Show Affiliations

Department of Comparative Anatomy and Pathological Anatomy, Faculty of Veterinary Science, University of Cordoba, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8888960

Citation

Rivero, J L., et al. "Myosin Heavy Chain Isoforms in Adult Equine Skeletal Muscle: an Immunohistochemical and Electrophoretic Study." The Anatomical Record, vol. 246, no. 2, 1996, pp. 185-94.
Rivero JL, Talmadge RJ, Edgerton VR. Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study. Anat Rec. 1996;246(2):185-94.
Rivero, J. L., Talmadge, R. J., & Edgerton, V. R. (1996). Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study. The Anatomical Record, 246(2), 185-94.
Rivero JL, Talmadge RJ, Edgerton VR. Myosin Heavy Chain Isoforms in Adult Equine Skeletal Muscle: an Immunohistochemical and Electrophoretic Study. Anat Rec. 1996;246(2):185-94. PubMed PMID: 8888960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study. AU - Rivero,J L, AU - Talmadge,R J, AU - Edgerton,V R, PY - 1996/10/1/pubmed PY - 2000/6/20/medline PY - 1996/10/1/entrez SP - 185 EP - 94 JF - The Anatomical record JO - Anat Rec VL - 246 IS - 2 N2 - BACKGROUND: The aim of this study was to characterize the myosin heavy chain (MyHC) isoforms present in equine skeletal muscle. METHODS: Muscle biopsies were removed from the superficial region of the gluteus medius muscle of five mature horses and analyzed by immunohistochemistry (using a battery of monoclonal antibodies specific for rat MyHC isoforms) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. RESULTS: Immunohistochemistry allowed subdivision of three different muscle fiber populations containing a single MyHC, one slow and two fast, and two hybrid populations, one containing slow and fast MyHCs and another with both fast-MyHC isoforms. Electrophoresis of MyHC confirmed the existence of three resolvable bands, with an electrophoretic mobility parallel to type I, IIa, and IIx rat MyHCs. The identities of two of these MyHCs were easily comparable with slow type I and fast type IIa MyHCs from rat skeletal muscle. However, a precise identification of the second fast MyHC was not made. CONCLUSIONS: These results show the presence of three different MyHC isoforms in mature equine skeletal muscle, whose differential distribution defines three fiber types containing a single MyHC and two hybrid fiber populations containing either both slow and fast type IIa MyHCs or both fast MyHC isoforms. SN - 0003-276X UR - https://www.unboundmedicine.com/medline/citation/8888960/Myosin_heavy_chain_isoforms_in_adult_equine_skeletal_muscle:_an_immunohistochemical_and_electrophoretic_study_ L2 - https://doi.org/10.1002/(SICI)1097-0185(199610)246:2<185::AID-AR5>3.0.CO;2-0 DB - PRIME DP - Unbound Medicine ER -