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A newly developed three-layer agarose microcapsule for a promising biohybrid artificial pancreas: rat to mouse xenotransplantation.
Cell Transplant. 1996 Sep-Oct; 5(5 Suppl 1):S59-63.CT

Abstract

We examined the effectiveness of an improved version of a three-layer agarose microcapsule in islet xenotransplantation. The microcapsule is composed of a mixture of 5% agarose and 5% polystyrene sulfonic acid. The other two outer layers are polybrene and carboxymethyl cellulose. The agarose/polystyrene sulfonic acid membrane is for the purpose of immunoisolation, suppression of complement activity and reinforcement of the microcapsule. The polybrene layer suppresses the polystyrene sulfonic acid leakage by forming a polyionic complex at the surface of the agarose/polystyrene sulfonic acid membrane. The outermost layer, a carboxymethyl cellulose coating, improves the biocompatibility of the microcapsule. In vitro static incubation study showed that the insulin secretion from rat islets in microcapsules in response to 16.7 mM glucose stimulation was more than four times higher than that on 3.3 mM glucose stimulation (n = 8). In an in vivo study, 500 rat islets in microcapsules were xenogenically implanted in the abdominal cavity of mice with streptozotocin-induced diabetes. The graft survival times ranged from 2 to 5 mo, the average being 75 days (n = 5). Our results demonstrate that the improved version of the three-layer agarose microcapsule can effectively prolong the xenograft survival time without employing immunosuppressants, suggesting that this microcapsule could provide a promising biohybrid artificial pancreas for future clinical applications.

Authors+Show Affiliations

First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8889234

Citation

Tun, T, et al. "A Newly Developed Three-layer Agarose Microcapsule for a Promising Biohybrid Artificial Pancreas: Rat to Mouse Xenotransplantation." Cell Transplantation, vol. 5, no. 5 Suppl 1, 1996, pp. S59-63.
Tun T, Inoue K, Hayashi H, et al. A newly developed three-layer agarose microcapsule for a promising biohybrid artificial pancreas: rat to mouse xenotransplantation. Cell Transplant. 1996;5(5 Suppl 1):S59-63.
Tun, T., Inoue, K., Hayashi, H., Aung, T., Gu, Y. J., Doi, R., Kaji, H., Echigo, Y., Wang, W. J., Setoyama, H., Imamura, M., Maetani, S., Morikawa, N., Iwata, H., & Ikada, Y. (1996). A newly developed three-layer agarose microcapsule for a promising biohybrid artificial pancreas: rat to mouse xenotransplantation. Cell Transplantation, 5(5 Suppl 1), S59-63.
Tun T, et al. A Newly Developed Three-layer Agarose Microcapsule for a Promising Biohybrid Artificial Pancreas: Rat to Mouse Xenotransplantation. Cell Transplant. 1996 Sep-Oct;5(5 Suppl 1):S59-63. PubMed PMID: 8889234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A newly developed three-layer agarose microcapsule for a promising biohybrid artificial pancreas: rat to mouse xenotransplantation. AU - Tun,T, AU - Inoue,K, AU - Hayashi,H, AU - Aung,T, AU - Gu,Y J, AU - Doi,R, AU - Kaji,H, AU - Echigo,Y, AU - Wang,W J, AU - Setoyama,H, AU - Imamura,M, AU - Maetani,S, AU - Morikawa,N, AU - Iwata,H, AU - Ikada,Y, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - S59 EP - 63 JF - Cell transplantation JO - Cell Transplant VL - 5 IS - 5 Suppl 1 N2 - We examined the effectiveness of an improved version of a three-layer agarose microcapsule in islet xenotransplantation. The microcapsule is composed of a mixture of 5% agarose and 5% polystyrene sulfonic acid. The other two outer layers are polybrene and carboxymethyl cellulose. The agarose/polystyrene sulfonic acid membrane is for the purpose of immunoisolation, suppression of complement activity and reinforcement of the microcapsule. The polybrene layer suppresses the polystyrene sulfonic acid leakage by forming a polyionic complex at the surface of the agarose/polystyrene sulfonic acid membrane. The outermost layer, a carboxymethyl cellulose coating, improves the biocompatibility of the microcapsule. In vitro static incubation study showed that the insulin secretion from rat islets in microcapsules in response to 16.7 mM glucose stimulation was more than four times higher than that on 3.3 mM glucose stimulation (n = 8). In an in vivo study, 500 rat islets in microcapsules were xenogenically implanted in the abdominal cavity of mice with streptozotocin-induced diabetes. The graft survival times ranged from 2 to 5 mo, the average being 75 days (n = 5). Our results demonstrate that the improved version of the three-layer agarose microcapsule can effectively prolong the xenograft survival time without employing immunosuppressants, suggesting that this microcapsule could provide a promising biohybrid artificial pancreas for future clinical applications. SN - 0963-6897 UR - https://www.unboundmedicine.com/medline/citation/8889234/A_newly_developed_three_layer_agarose_microcapsule_for_a_promising_biohybrid_artificial_pancreas:_rat_to_mouse_xenotransplantation_ L2 - https://journals.sagepub.com/doi/10.1016/0963-6897(96)00042-5?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -