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Quantitative determination of phencyclidine in pigmented and nonpigmented hair by ion-trap mass spectrometry.
J Anal Toxicol. 1996 Oct; 20(6):350-4.JA

Abstract

A sensitive and specific method has been developed for the quantitative analysis of phencyclidine (PCP) in pigmented and nonpigmented rat hair. After the addition of PCP-d5 as the internal standard, hair samples (10 mg) were digested overnight in 1N NaOH at 30 degrees C. Digested solutions were then extracted using a solid-phase procedure with Bond Elut CertifyTM extraction columns. Reconstituted extracts were analyzed on a Finnigan ion trap (MagnumTM) mass spectrometer in the electron ionization mode using helium as the carrier gas, and a DB-5 MS (30 m x 0.25-mm i.d.; 25-microns film thickness) capillary column. The assay is linear from 0.1 to 50 ng/mg with a correlation coefficient of > 0.99 and is capable of detecting 25 pg of PCP on column. The accuracy of this assay was estimated using fortified hair standards at PCP concentrations of 0.5 and 10 ng/mg. Intra-assay coefficients of variation were determined to be less than 6% at 0.5, 2, and 10 ng/mg. Interassay coefficients of variation were determined to be less than 15% at 0.5, 2, and 10 ng/mg. The method has been used to evaluate PCP incorporation into Long-Evans rat hair but could also be used to evaluate the incorporation of PCP into human hair. Male rats were shaved prior to dosing such that both pigmented and nonpigmented hair was collected. Animals were administered 12 mg/kg PCP by intraperitoneal injection daily for five days. Fourteen days after the first dose, pigmented and nonpigmented hair were collected and analyzed for PCP. The mean plus or minus the standard error of the mean (n = 5) concentrations of PCP in pigmented and nonpigmented hair were 14.33 +/- 1.43 ng/mg of hair and 0.47 +/- 0.04 ng/mg of hair, respectively. This method is also being used to evaluate PCP as a model xenobiotic for studies of the incorporation of xenobiotics into hair.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 84112, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8889669

Citation

Slawson, M H., et al. "Quantitative Determination of Phencyclidine in Pigmented and Nonpigmented Hair By Ion-trap Mass Spectrometry." Journal of Analytical Toxicology, vol. 20, no. 6, 1996, pp. 350-4.
Slawson MH, Wilkins DG, Foltz RL, et al. Quantitative determination of phencyclidine in pigmented and nonpigmented hair by ion-trap mass spectrometry. J Anal Toxicol. 1996;20(6):350-4.
Slawson, M. H., Wilkins, D. G., Foltz, R. L., & Rollins, D. E. (1996). Quantitative determination of phencyclidine in pigmented and nonpigmented hair by ion-trap mass spectrometry. Journal of Analytical Toxicology, 20(6), 350-4.
Slawson MH, et al. Quantitative Determination of Phencyclidine in Pigmented and Nonpigmented Hair By Ion-trap Mass Spectrometry. J Anal Toxicol. 1996;20(6):350-4. PubMed PMID: 8889669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination of phencyclidine in pigmented and nonpigmented hair by ion-trap mass spectrometry. AU - Slawson,M H, AU - Wilkins,D G, AU - Foltz,R L, AU - Rollins,D E, PY - 1996/10/1/pubmed PY - 1996/10/1/medline PY - 1996/10/1/entrez SP - 350 EP - 4 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 20 IS - 6 N2 - A sensitive and specific method has been developed for the quantitative analysis of phencyclidine (PCP) in pigmented and nonpigmented rat hair. After the addition of PCP-d5 as the internal standard, hair samples (10 mg) were digested overnight in 1N NaOH at 30 degrees C. Digested solutions were then extracted using a solid-phase procedure with Bond Elut CertifyTM extraction columns. Reconstituted extracts were analyzed on a Finnigan ion trap (MagnumTM) mass spectrometer in the electron ionization mode using helium as the carrier gas, and a DB-5 MS (30 m x 0.25-mm i.d.; 25-microns film thickness) capillary column. The assay is linear from 0.1 to 50 ng/mg with a correlation coefficient of > 0.99 and is capable of detecting 25 pg of PCP on column. The accuracy of this assay was estimated using fortified hair standards at PCP concentrations of 0.5 and 10 ng/mg. Intra-assay coefficients of variation were determined to be less than 6% at 0.5, 2, and 10 ng/mg. Interassay coefficients of variation were determined to be less than 15% at 0.5, 2, and 10 ng/mg. The method has been used to evaluate PCP incorporation into Long-Evans rat hair but could also be used to evaluate the incorporation of PCP into human hair. Male rats were shaved prior to dosing such that both pigmented and nonpigmented hair was collected. Animals were administered 12 mg/kg PCP by intraperitoneal injection daily for five days. Fourteen days after the first dose, pigmented and nonpigmented hair were collected and analyzed for PCP. The mean plus or minus the standard error of the mean (n = 5) concentrations of PCP in pigmented and nonpigmented hair were 14.33 +/- 1.43 ng/mg of hair and 0.47 +/- 0.04 ng/mg of hair, respectively. This method is also being used to evaluate PCP as a model xenobiotic for studies of the incorporation of xenobiotics into hair. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/8889669/Quantitative_determination_of_phencyclidine_in_pigmented_and_nonpigmented_hair_by_ion_trap_mass_spectrometry_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/20.6.350 DB - PRIME DP - Unbound Medicine ER -