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Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51.
Infect Immun 1996; 64(11):4534-41II

Abstract

Immune responses and resistance to infection with Brucella abortus 2308 (S2308) were measured in mice following oral or intraperitoneal (i.p.) vaccination with strain RB51 (SRB51). Bacteria persisted in the parotid lymph node for 4 weeks following oral vaccination of mice with 5 x 10(8) or 5 x 10(6) CFU of SRB51. Bacteria did not appear in the spleen during 12 weeks after oral vaccination, whereas they did appear in the spleen for 8 weeks following i.p. vaccination of mice with SRB51 (5 x 10(8) or 5 x 10(6) CFU). Increased resistance to S2308 infection occurred at 12 to 20 weeks in mice vaccinated i.p. with SRB51 (5 x 10(8) or 5 x 10(6) CFU) but occurred at 12 weeks only in mice vaccinated orally with SRB51 (5 x 10(8) CFU). Oral SRB51 vaccination induced lower levels of antibodies to the surface antigens of intact SRB51 bacteria than did i.p. vaccination. However, neither route of vaccination induced anamnestic antibody responses to the surface antigens of intact S2308 bacteria after challenge infection of the vaccinated mice with S2308. Mice vaccinated orally with SRB51 and challenged with S2308 at 12 to 20 weeks had lower and less persistent spleen cell proliferation and production of gamma interferon in response to S2308 and certain immunodominant S2308 proteins (32 to < or = 18 kDa) than did mice vaccinated i.p. with SRB51. However, mice vaccinated orally or i.p. with SRB51 and challenged with S2308 had similar spleen cell tumor necrosis factor alpha production. These results indicate that oral vaccination of mice with SRB51 was effective in inducing protective immunity to S2308 infection, although the immunity was lower and less persistent than that induced by i.p. vaccination. The lower protective immunity induced by oral vaccination may have resulted from lower and less persistent cell-mediated immunity and gamma interferon production in response to S2308 and S2308 proteins.

Authors+Show Affiliations

Zoonotic Diseases Research Unit, National Animal Disease Center, USDA Agriculture Research Service, Ames, Iowa 50010, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8890203

Citation

Stevens, M G., et al. "Immune Responses and Resistance to Brucellosis in Mice Vaccinated Orally With Brucella Abortus RB51." Infection and Immunity, vol. 64, no. 11, 1996, pp. 4534-41.
Stevens MG, Olsen SC, Palmer MV, et al. Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51. Infect Immun. 1996;64(11):4534-41.
Stevens, M. G., Olsen, S. C., Palmer, M. V., & Pugh, G. W. (1996). Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51. Infection and Immunity, 64(11), pp. 4534-41.
Stevens MG, et al. Immune Responses and Resistance to Brucellosis in Mice Vaccinated Orally With Brucella Abortus RB51. Infect Immun. 1996;64(11):4534-41. PubMed PMID: 8890203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51. AU - Stevens,M G, AU - Olsen,S C, AU - Palmer,M V, AU - Pugh,G W,Jr PY - 1996/11/1/pubmed PY - 2001/3/28/medline PY - 1996/11/1/entrez SP - 4534 EP - 41 JF - Infection and immunity JO - Infect. Immun. VL - 64 IS - 11 N2 - Immune responses and resistance to infection with Brucella abortus 2308 (S2308) were measured in mice following oral or intraperitoneal (i.p.) vaccination with strain RB51 (SRB51). Bacteria persisted in the parotid lymph node for 4 weeks following oral vaccination of mice with 5 x 10(8) or 5 x 10(6) CFU of SRB51. Bacteria did not appear in the spleen during 12 weeks after oral vaccination, whereas they did appear in the spleen for 8 weeks following i.p. vaccination of mice with SRB51 (5 x 10(8) or 5 x 10(6) CFU). Increased resistance to S2308 infection occurred at 12 to 20 weeks in mice vaccinated i.p. with SRB51 (5 x 10(8) or 5 x 10(6) CFU) but occurred at 12 weeks only in mice vaccinated orally with SRB51 (5 x 10(8) CFU). Oral SRB51 vaccination induced lower levels of antibodies to the surface antigens of intact SRB51 bacteria than did i.p. vaccination. However, neither route of vaccination induced anamnestic antibody responses to the surface antigens of intact S2308 bacteria after challenge infection of the vaccinated mice with S2308. Mice vaccinated orally with SRB51 and challenged with S2308 at 12 to 20 weeks had lower and less persistent spleen cell proliferation and production of gamma interferon in response to S2308 and certain immunodominant S2308 proteins (32 to < or = 18 kDa) than did mice vaccinated i.p. with SRB51. However, mice vaccinated orally or i.p. with SRB51 and challenged with S2308 had similar spleen cell tumor necrosis factor alpha production. These results indicate that oral vaccination of mice with SRB51 was effective in inducing protective immunity to S2308 infection, although the immunity was lower and less persistent than that induced by i.p. vaccination. The lower protective immunity induced by oral vaccination may have resulted from lower and less persistent cell-mediated immunity and gamma interferon production in response to S2308 and S2308 proteins. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/8890203/Immune_responses_and_resistance_to_brucellosis_in_mice_vaccinated_orally_with_Brucella_abortus_RB51_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&amp;pmid=8890203 DB - PRIME DP - Unbound Medicine ER -