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Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse.
Infect Immun. 1996 Nov; 64(11):4549-56.II

Abstract

We examined the role of iron(II) and iron(III) uptake, mediated by FeoB and TonB, respectively, in infection of the mouse by Salmonella typhimurium. The S. typhimurium feoB gene, encoding a homolog of an Escherichia coli cytoplasmic membrane iron(II) permease, was cloned, and a mutant was generated by allelic exchange. In addition, an S. typhimurium tonB mutant was constructed. Together these two mutations inactivate all known iron uptake systems of S. typhimurium. We examined the abilities of these mutants to grow in vitro and in different compartments of the host. Mutants in feoB were outcompeted by the wild type during mixed colonization of the mouse intestine, but the feoB mutation did not attenuate S. typhimurium for oral or intraperitoneal infection of mice. The tonB mutation attenuated S. typhimurium for infection of mice by the intragastric route but not the intraperitoneal route, and the mutant was recovered in lower numbers from the Peyer's patches and mesenteric lymph nodes than the wild type. These results indicate that TonB-mediated iron uptake contributes to colonization of the Peyer's patches and mesenteric lymph nodes but not the liver and spleen of the mouse. The tonB feoB double mutant, given intraperitoneally, was able to infect the liver and spleen at wild-type doses, indicating that additional iron acquisition systems are used during growth at systemic sites of infection.

Authors+Show Affiliations

Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8890205

Citation

Tsolis, R M., et al. "Contribution of TonB- and Feo-mediated Iron Uptake to Growth of Salmonella Typhimurium in the Mouse." Infection and Immunity, vol. 64, no. 11, 1996, pp. 4549-56.
Tsolis RM, Bäumler AJ, Heffron F, et al. Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse. Infect Immun. 1996;64(11):4549-56.
Tsolis, R. M., Bäumler, A. J., Heffron, F., & Stojiljkovic, I. (1996). Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse. Infection and Immunity, 64(11), 4549-56.
Tsolis RM, et al. Contribution of TonB- and Feo-mediated Iron Uptake to Growth of Salmonella Typhimurium in the Mouse. Infect Immun. 1996;64(11):4549-56. PubMed PMID: 8890205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contribution of TonB- and Feo-mediated iron uptake to growth of Salmonella typhimurium in the mouse. AU - Tsolis,R M, AU - Bäumler,A J, AU - Heffron,F, AU - Stojiljkovic,I, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 4549 EP - 56 JF - Infection and immunity JO - Infect Immun VL - 64 IS - 11 N2 - We examined the role of iron(II) and iron(III) uptake, mediated by FeoB and TonB, respectively, in infection of the mouse by Salmonella typhimurium. The S. typhimurium feoB gene, encoding a homolog of an Escherichia coli cytoplasmic membrane iron(II) permease, was cloned, and a mutant was generated by allelic exchange. In addition, an S. typhimurium tonB mutant was constructed. Together these two mutations inactivate all known iron uptake systems of S. typhimurium. We examined the abilities of these mutants to grow in vitro and in different compartments of the host. Mutants in feoB were outcompeted by the wild type during mixed colonization of the mouse intestine, but the feoB mutation did not attenuate S. typhimurium for oral or intraperitoneal infection of mice. The tonB mutation attenuated S. typhimurium for infection of mice by the intragastric route but not the intraperitoneal route, and the mutant was recovered in lower numbers from the Peyer's patches and mesenteric lymph nodes than the wild type. These results indicate that TonB-mediated iron uptake contributes to colonization of the Peyer's patches and mesenteric lymph nodes but not the liver and spleen of the mouse. The tonB feoB double mutant, given intraperitoneally, was able to infect the liver and spleen at wild-type doses, indicating that additional iron acquisition systems are used during growth at systemic sites of infection. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/8890205/Contribution_of_TonB__and_Feo_mediated_iron_uptake_to_growth_of_Salmonella_typhimurium_in_the_mouse_ L2 - https://journals.asm.org/doi/10.1128/iai.64.11.4549-4556.1996?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -