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Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes.
J Immunol. 1996 Nov 01; 157(9):3850-9.JI

Abstract

Dendritic cells (DC) are the most potent APCs within the immune system. We show here that highly purified CD14(bright) peripheral blood monocytes supplemented with granulocyte-monocyte (GM)-CSF plus IL-4 develop with high efficacy (>95% of input cells) into DC. They neo-expressed CD1a, CD1b, CD1c, CD80, and CD5; they massively up-regulated CD40 (109-fold) and HLA-DQ and DP (125- and 87-fold); and significantly (>5-fold) up-regulated HLA-DR, CD4, CD11b, CD11c, CD43, CD45, CD45R0, CD54, CD58, and CD59. CD14, CD15s, CD64, and CDw65 molecules were down-regulated to background levels, and no major changes were observed for HLA class I, CD11a, CD32, CD33, CD48, CD50, CD86, CDw92, CD93, or CD97. Monocytes cultured in parallel with GM-CSF plus TNF-alpha were more heterogeneous in expression densities but otherwise similar in their surface molecule repertoire. They clearly differed, however, in their accessory cell capacity. Only GM-CSF plus IL-4-cultured cells were found to be potent stimulators in allogeneic and autologous MLR and they presented tetanus toxoid 100- to 1000-fold more efficiently than other cell populations tested. Furthermore, only cytokine-treated monocytes formed clusters with resting T cells. At variance from all these similarities between in vitro-generated monocyte-derived DC and in vivo-developing DC, the DC populations generated by us contained significant amounts of myeloperoxidase and also expressed lysozyme. At least in this respect they, thus, differ from "classical" DC types.

Authors+Show Affiliations

Institute of Immunology, University of Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8892615

Citation

Pickl, W F., et al. "Molecular and Functional Characteristics of Dendritic Cells Generated From Highly Purified CD14+ Peripheral Blood Monocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 157, no. 9, 1996, pp. 3850-9.
Pickl WF, Majdic O, Kohl P, et al. Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes. J Immunol. 1996;157(9):3850-9.
Pickl, W. F., Majdic, O., Kohl, P., Stöckl, J., Riedl, E., Scheinecker, C., Bello-Fernandez, C., & Knapp, W. (1996). Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes. Journal of Immunology (Baltimore, Md. : 1950), 157(9), 3850-9.
Pickl WF, et al. Molecular and Functional Characteristics of Dendritic Cells Generated From Highly Purified CD14+ Peripheral Blood Monocytes. J Immunol. 1996 Nov 1;157(9):3850-9. PubMed PMID: 8892615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and functional characteristics of dendritic cells generated from highly purified CD14+ peripheral blood monocytes. AU - Pickl,W F, AU - Majdic,O, AU - Kohl,P, AU - Stöckl,J, AU - Riedl,E, AU - Scheinecker,C, AU - Bello-Fernandez,C, AU - Knapp,W, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 3850 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 157 IS - 9 N2 - Dendritic cells (DC) are the most potent APCs within the immune system. We show here that highly purified CD14(bright) peripheral blood monocytes supplemented with granulocyte-monocyte (GM)-CSF plus IL-4 develop with high efficacy (>95% of input cells) into DC. They neo-expressed CD1a, CD1b, CD1c, CD80, and CD5; they massively up-regulated CD40 (109-fold) and HLA-DQ and DP (125- and 87-fold); and significantly (>5-fold) up-regulated HLA-DR, CD4, CD11b, CD11c, CD43, CD45, CD45R0, CD54, CD58, and CD59. CD14, CD15s, CD64, and CDw65 molecules were down-regulated to background levels, and no major changes were observed for HLA class I, CD11a, CD32, CD33, CD48, CD50, CD86, CDw92, CD93, or CD97. Monocytes cultured in parallel with GM-CSF plus TNF-alpha were more heterogeneous in expression densities but otherwise similar in their surface molecule repertoire. They clearly differed, however, in their accessory cell capacity. Only GM-CSF plus IL-4-cultured cells were found to be potent stimulators in allogeneic and autologous MLR and they presented tetanus toxoid 100- to 1000-fold more efficiently than other cell populations tested. Furthermore, only cytokine-treated monocytes formed clusters with resting T cells. At variance from all these similarities between in vitro-generated monocyte-derived DC and in vivo-developing DC, the DC populations generated by us contained significant amounts of myeloperoxidase and also expressed lysozyme. At least in this respect they, thus, differ from "classical" DC types. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8892615/Molecular_and_functional_characteristics_of_dendritic_cells_generated_from_highly_purified_CD14+_peripheral_blood_monocytes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8892615 DB - PRIME DP - Unbound Medicine ER -