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Regulation by IL-5 of expression of functional platelet-activating factor receptors on human eosinophils.
J Immunol. 1996 Nov 01; 157(9):4126-32.JI

Abstract

Platelet-activating factor (PAF) and IL-5 are both important mediators of various allergic reactions, although the relationship between these mediators in allergic responses has not yet been fully elucidated. In this study, we investigated the effects of human rIL-5 on the expression of the PAF receptor (PAF-R) on eosinophils from healthy human volunteers. The specific binding of [3H]WEB 2086, a specific ligand for PAF-R, to eosinophils treated with 5 ng/ml of IL-5 for 12 h was significantly higher than that to untreated cells. The Bmax value for IL-5-treated eosinophils was 1.8-fold higher than that for untreated cells, while the Kd values remained unchanged. This clearly indicates that surface expression of PAF-R on eosinophils is enhanced by IL-5. The enhancement was first observed at 6 h and reached a plateau at 12 to 18 h. The effect of IL-5 was abolished in the presence of cycloheximide or actinomycin D. When the relative amount of PAF-R mRNA was determined by reverse transcription PCR, the message was found to be increased through activation of transcription of transcript 1 on exposure to IL-5. Furthermore, we found that the PAF-induced increase in intracellular calcium ion concentration in eosinophils was markedly augmented by exposure to IL-5 for 12 h. Several lines of data suggest that the enhanced expression of PAF-R on eosinophils due to IL-5 is the cause of the augmented response to PAF. These findings will be of value for understanding the mechanisms of selective infiltration and activation of eosinophils in allergic diseases in which PAF and IL-5 are crucially involved.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8892648

Citation

Kishimoto, S, et al. "Regulation By IL-5 of Expression of Functional Platelet-activating Factor Receptors On Human Eosinophils." Journal of Immunology (Baltimore, Md. : 1950), vol. 157, no. 9, 1996, pp. 4126-32.
Kishimoto S, Shimadzu W, Izumi T, et al. Regulation by IL-5 of expression of functional platelet-activating factor receptors on human eosinophils. J Immunol. 1996;157(9):4126-32.
Kishimoto, S., Shimadzu, W., Izumi, T., Shimizu, T., Fukuda, T., Makino, S., Sugiura, T., & Waku, K. (1996). Regulation by IL-5 of expression of functional platelet-activating factor receptors on human eosinophils. Journal of Immunology (Baltimore, Md. : 1950), 157(9), 4126-32.
Kishimoto S, et al. Regulation By IL-5 of Expression of Functional Platelet-activating Factor Receptors On Human Eosinophils. J Immunol. 1996 Nov 1;157(9):4126-32. PubMed PMID: 8892648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation by IL-5 of expression of functional platelet-activating factor receptors on human eosinophils. AU - Kishimoto,S, AU - Shimadzu,W, AU - Izumi,T, AU - Shimizu,T, AU - Fukuda,T, AU - Makino,S, AU - Sugiura,T, AU - Waku,K, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 4126 EP - 32 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 157 IS - 9 N2 - Platelet-activating factor (PAF) and IL-5 are both important mediators of various allergic reactions, although the relationship between these mediators in allergic responses has not yet been fully elucidated. In this study, we investigated the effects of human rIL-5 on the expression of the PAF receptor (PAF-R) on eosinophils from healthy human volunteers. The specific binding of [3H]WEB 2086, a specific ligand for PAF-R, to eosinophils treated with 5 ng/ml of IL-5 for 12 h was significantly higher than that to untreated cells. The Bmax value for IL-5-treated eosinophils was 1.8-fold higher than that for untreated cells, while the Kd values remained unchanged. This clearly indicates that surface expression of PAF-R on eosinophils is enhanced by IL-5. The enhancement was first observed at 6 h and reached a plateau at 12 to 18 h. The effect of IL-5 was abolished in the presence of cycloheximide or actinomycin D. When the relative amount of PAF-R mRNA was determined by reverse transcription PCR, the message was found to be increased through activation of transcription of transcript 1 on exposure to IL-5. Furthermore, we found that the PAF-induced increase in intracellular calcium ion concentration in eosinophils was markedly augmented by exposure to IL-5 for 12 h. Several lines of data suggest that the enhanced expression of PAF-R on eosinophils due to IL-5 is the cause of the augmented response to PAF. These findings will be of value for understanding the mechanisms of selective infiltration and activation of eosinophils in allergic diseases in which PAF and IL-5 are crucially involved. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8892648/Regulation_by_IL_5_of_expression_of_functional_platelet_activating_factor_receptors_on_human_eosinophils_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8892648 DB - PRIME DP - Unbound Medicine ER -