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Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica.
Arch Microbiol. 1977 May 13; 113(1-2):99-104.AM

Abstract

The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the same time production of acids begins. The activity of the NAD-dependent isocitrate dehydrogenase which requires AMP for activity becomes very low and prevents the oxidative function of the citric acid cycle whereas isocitrate lyase is not inhibited. As citrate synthase inhibition by ATP appears to be insufficient to stop n-paraffin degradation, citric and isocitric acids accumulation can take place. Massive excretion of these acids, however, probably still involves other physiological changes brought about by nitrogen limitation, possibly some permeabilization of the cell to these acids.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

889390

Citation

Marchal, R, et al. "Regulation of the Central Metabolism in Relation to Citric Acid Production in Saccharomycopsis Lipolytica." Archives of Microbiology, vol. 113, no. 1-2, 1977, pp. 99-104.
Marchal R, Vandecasteele JP, Metche M. Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica. Arch Microbiol. 1977;113(1-2):99-104.
Marchal, R., Vandecasteele, J. P., & Metche, M. (1977). Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica. Archives of Microbiology, 113(1-2), 99-104.
Marchal R, Vandecasteele JP, Metche M. Regulation of the Central Metabolism in Relation to Citric Acid Production in Saccharomycopsis Lipolytica. Arch Microbiol. 1977 May 13;113(1-2):99-104. PubMed PMID: 889390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica. AU - Marchal,R, AU - Vandecasteele,J P, AU - Metche,M, PY - 1977/5/13/pubmed PY - 1977/5/13/medline PY - 1977/5/13/entrez SP - 99 EP - 104 JF - Archives of microbiology JO - Arch Microbiol VL - 113 IS - 1-2 N2 - The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the same time production of acids begins. The activity of the NAD-dependent isocitrate dehydrogenase which requires AMP for activity becomes very low and prevents the oxidative function of the citric acid cycle whereas isocitrate lyase is not inhibited. As citrate synthase inhibition by ATP appears to be insufficient to stop n-paraffin degradation, citric and isocitric acids accumulation can take place. Massive excretion of these acids, however, probably still involves other physiological changes brought about by nitrogen limitation, possibly some permeabilization of the cell to these acids. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/889390/Regulation_of_the_central_metabolism_in_relation_to_citric_acid_production_in_Saccharomycopsis_lipolytica_ L2 - https://antibodies.cancer.gov/detail/CPTC-HLA-B-1 DB - PRIME DP - Unbound Medicine ER -