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Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis.
J Clin Microbiol. 1996 Nov; 34(11):2826-8.JC

Abstract

Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results.

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Authors+Show Affiliations

Tanaka K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Miyazaki T
No affiliation info available
Maesaki S
No affiliation info available
Mitsutake K
No affiliation info available
Kakeya H
No affiliation info available
Yamamoto Y
No affiliation info available
Yanagihara K
No affiliation info available
Hossain MA
No affiliation info available
Tashiro T
No affiliation info available
Kohno S
No affiliation info available

MeSH

Base SequenceCryptococcosisCryptococcus neoformansDNA PrimersDNA, FungalDiagnostic ErrorsEvaluation Studies as TopicGenes, FungalHumansLung Diseases, FungalMycologyPolymerase Chain Reaction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8897191

Citation

Tanaka, K, et al. "Detection of Cryptococcus Neoformans Gene in Patients With Pulmonary Cryptococcosis." Journal of Clinical Microbiology, vol. 34, no. 11, 1996, pp. 2826-8.
Tanaka K, Miyazaki T, Maesaki S, et al. Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis. J Clin Microbiol. 1996;34(11):2826-8.
Tanaka, K., Miyazaki, T., Maesaki, S., Mitsutake, K., Kakeya, H., Yamamoto, Y., Yanagihara, K., Hossain, M. A., Tashiro, T., & Kohno, S. (1996). Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis. Journal of Clinical Microbiology, 34(11), 2826-8.
Tanaka K, et al. Detection of Cryptococcus Neoformans Gene in Patients With Pulmonary Cryptococcosis. J Clin Microbiol. 1996;34(11):2826-8. PubMed PMID: 8897191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Cryptococcus neoformans gene in patients with pulmonary cryptococcosis. AU - Tanaka,K, AU - Miyazaki,T, AU - Maesaki,S, AU - Mitsutake,K, AU - Kakeya,H, AU - Yamamoto,Y, AU - Yanagihara,K, AU - Hossain,M A, AU - Tashiro,T, AU - Kohno,S, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 2826 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 34 IS - 11 N2 - Pulmonary cryptococcosis was diagnosed by nested PCR. Extraction of DNA was performed by mechanical destruction of the capsules of Cryptococcus neoformans by the glass bead technique. Nested PCR was positive for 4 of 5 culture-positive specimens but negative for 1 culture-positive specimen, 10 culture-negative specimens, and 1 specimen with undetermined culture results. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/8897191/Detection_of_Cryptococcus_neoformans_gene_in_patients_with_pulmonary_cryptococcosis_ DB - PRIME DP - Unbound Medicine ER -