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K(86Rb) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells.
Am J Physiol 1996; 271(4 Pt 1):C1111-21AJ

Abstract

Previous studies have suggested ion transport heterogeneity among sickle cell anemia (SS) reticulocytes that could influence their dehydration susceptibility. We examined Ca2(+)-independent K transport in the lowest density (F1), reticulocyte-rich SS cells, measuring the effects of acidification, ouabain, and bumetanide on their unidirectional K(86Rb) fluxes. Unlike those of normal red blood cells and SS discocytes, the SS-F1 K(86Rb) fluxes were highly nonlinear, with large 5-min flux components (previously unobserved) and a more gradual decline over 60 min. Analysis revealed two distinct K pools: a rapid-turnover pool in a small fraction of cells, whose major ouabain-resistant K(86Rb) transport path showed distinctive properties including inhibition by high concentrations of bumetanide (> or = 1 mM) and stimulation at pH 7.0, and another heterogeneous, relatively slow-turnover pool, in most of the F1 cells, whose main ouabain-resistant K(86Rb) path was insensitive to bumetanide but was stimulated at pH 7.0, which is consistent with heterogeneous expression of the acid-sensitive K-Cl cotransport and with both rapid and slower generation of dehydrated SS cells.

Authors+Show Affiliations

Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8897817

Citation

Etzion, Z, et al. "K(86Rb) Transport Heterogeneity in the Low-density Fraction of Sickle Cell Anemia Red Blood Cells." The American Journal of Physiology, vol. 271, no. 4 Pt 1, 1996, pp. C1111-21.
Etzion Z, Lew VL, Bookchin RM. K(86Rb) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells. Am J Physiol. 1996;271(4 Pt 1):C1111-21.
Etzion, Z., Lew, V. L., & Bookchin, R. M. (1996). K(86Rb) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells. The American Journal of Physiology, 271(4 Pt 1), pp. C1111-21.
Etzion Z, Lew VL, Bookchin RM. K(86Rb) Transport Heterogeneity in the Low-density Fraction of Sickle Cell Anemia Red Blood Cells. Am J Physiol. 1996;271(4 Pt 1):C1111-21. PubMed PMID: 8897817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - K(86Rb) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells. AU - Etzion,Z, AU - Lew,V L, AU - Bookchin,R M, PY - 1996/10/1/pubmed PY - 1996/10/1/medline PY - 1996/10/1/entrez SP - C1111 EP - 21 JF - The American journal of physiology JO - Am. J. Physiol. VL - 271 IS - 4 Pt 1 N2 - Previous studies have suggested ion transport heterogeneity among sickle cell anemia (SS) reticulocytes that could influence their dehydration susceptibility. We examined Ca2(+)-independent K transport in the lowest density (F1), reticulocyte-rich SS cells, measuring the effects of acidification, ouabain, and bumetanide on their unidirectional K(86Rb) fluxes. Unlike those of normal red blood cells and SS discocytes, the SS-F1 K(86Rb) fluxes were highly nonlinear, with large 5-min flux components (previously unobserved) and a more gradual decline over 60 min. Analysis revealed two distinct K pools: a rapid-turnover pool in a small fraction of cells, whose major ouabain-resistant K(86Rb) transport path showed distinctive properties including inhibition by high concentrations of bumetanide (> or = 1 mM) and stimulation at pH 7.0, and another heterogeneous, relatively slow-turnover pool, in most of the F1 cells, whose main ouabain-resistant K(86Rb) path was insensitive to bumetanide but was stimulated at pH 7.0, which is consistent with heterogeneous expression of the acid-sensitive K-Cl cotransport and with both rapid and slower generation of dehydrated SS cells. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/8897817/K_86Rb__transport_heterogeneity_in_the_low_density_fraction_of_sickle_cell_anemia_red_blood_cells_ L2 - http://www.physiology.org/doi/full/10.1152/ajpcell.1996.271.4.C1111?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -