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Identification and characterization of intrinsic factor and cobalophilin from pig ileal and pyloric mucosa.
Biochim Biophys Acta. 1977 May 26; 497(3):663-72.BB

Abstract

Pig ileal mucosa was found to bind about 240 ng vitamin B12/g and to contain two vitamin B12-binding proteins. One was highly active in the Schilling test, behaved immunologically as intrinsic factor and was responsible for about half of the total vitamin B12-binding capacity. The other binder was identified as cobalophilin (R-protein). Immunochemical purification of these proteins from pig ileum and pylorus was performed and the molecular characteristics (sedimentation and diffusion coefficients, Stokes radii, frictional ratios and molecular weights) of their vitamin B12 complexes were estimated. Isoelectric focusing revealed differences between the ileal and pyloric intrinsic factors but not between the cobalophilins. The mean isoelectric points of the pyloric and ileal intrinsic factors were pH 5979 and 5.30, respectively, whereas the corresponding figures for the cobalophilins were 4.13 and 4.10.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

889882

Citation

Marcoullis, G, et al. "Identification and Characterization of Intrinsic Factor and Cobalophilin From Pig Ileal and Pyloric Mucosa." Biochimica Et Biophysica Acta, vol. 497, no. 3, 1977, pp. 663-72.
Marcoullis G, Gräsbeck R, Salonen EM. Identification and characterization of intrinsic factor and cobalophilin from pig ileal and pyloric mucosa. Biochim Biophys Acta. 1977;497(3):663-72.
Marcoullis, G., Gräsbeck, R., & Salonen, E. M. (1977). Identification and characterization of intrinsic factor and cobalophilin from pig ileal and pyloric mucosa. Biochimica Et Biophysica Acta, 497(3), 663-72.
Marcoullis G, Gräsbeck R, Salonen EM. Identification and Characterization of Intrinsic Factor and Cobalophilin From Pig Ileal and Pyloric Mucosa. Biochim Biophys Acta. 1977 May 26;497(3):663-72. PubMed PMID: 889882.
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TY - JOUR T1 - Identification and characterization of intrinsic factor and cobalophilin from pig ileal and pyloric mucosa. AU - Marcoullis,G, AU - Gräsbeck,R, AU - Salonen,E M, PY - 1977/5/26/pubmed PY - 1977/5/26/medline PY - 1977/5/26/entrez SP - 663 EP - 72 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 497 IS - 3 N2 - Pig ileal mucosa was found to bind about 240 ng vitamin B12/g and to contain two vitamin B12-binding proteins. One was highly active in the Schilling test, behaved immunologically as intrinsic factor and was responsible for about half of the total vitamin B12-binding capacity. The other binder was identified as cobalophilin (R-protein). Immunochemical purification of these proteins from pig ileum and pylorus was performed and the molecular characteristics (sedimentation and diffusion coefficients, Stokes radii, frictional ratios and molecular weights) of their vitamin B12 complexes were estimated. Isoelectric focusing revealed differences between the ileal and pyloric intrinsic factors but not between the cobalophilins. The mean isoelectric points of the pyloric and ileal intrinsic factors were pH 5979 and 5.30, respectively, whereas the corresponding figures for the cobalophilins were 4.13 and 4.10. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/889882/Identification_and_characterization_of_intrinsic_factor_and_cobalophilin_from_pig_ileal_and_pyloric_mucosa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-4165(77)90287-2 DB - PRIME DP - Unbound Medicine ER -