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Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro.
Eur J Immunol. 1996 Oct; 26(10):2371-5.EJ

Abstract

A mouse monoclonal antibody (mAb) to the rat interleukin-2 receptor beta (IL-2R beta) chain was generated using IL-2R beta cDNA-transfected mouse L929 cells for immunization and differential screening. This antibody, called L316, detects a cell surface protein with an apparent molecular mass of about 80 kDa. In peripheral lymphoid organs of young adult rats, IL-2R beta expression is restricted to T and natural killer (NK) cells, and less than 10% of IL-2R beta+ cells co-express the IL-2R alpha chain. IL-2R beta was detected on all NKRP-1hi (NK-) and NKRP-1lo cells (T-lineage cells of unknown function), most peripheral gama delta T cells and on 30-40 % of CD8 and 10 % of CD4 alpha beta T cells. In the adult rat thymus, mAb L316 detects a small subset (about 1%) of predominantly IL-2R alpha- cells which express cell surface markers characteristic of mature T lymphocytes and contain a high proportion of CD4-8- and CD4-8+ alpha beta T cell receptor (TCR)+ thymocytes. TCR-V usage suggests that major histocompatibility complex (MHC) class I plays a more important role than MHC class II in the selection of these cells. On immature CD4+8+ rat thymocytes, IL-2R beta cell surface expression is readily induced by TCR stimulation in vitro, supporting the idea that in vivo, the IL-2R beta+ phenotype is the the result of TCR engagement during thymic selection.

Authors+Show Affiliations

Institute for Virology and Immunobiology, University of Würzburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8898947

Citation

Park, J H., et al. "Identification and Cellular Distribution of the Rat Interleukin-2 Receptor Beta Chain: Induction of the IL-2R Alpha- Beta+ Phenotype By Major Histocompatibility Complex Class I Recognition During T Cell Development in Vivo and By T Cell Receptor Stimulation of CD4+8+ Immature Thymocytes in Vitro." European Journal of Immunology, vol. 26, no. 10, 1996, pp. 2371-5.
Park JH, Hanke T, Hünig T. Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro. Eur J Immunol. 1996;26(10):2371-5.
Park, J. H., Hanke, T., & Hünig, T. (1996). Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro. European Journal of Immunology, 26(10), 2371-5.
Park JH, Hanke T, Hünig T. Identification and Cellular Distribution of the Rat Interleukin-2 Receptor Beta Chain: Induction of the IL-2R Alpha- Beta+ Phenotype By Major Histocompatibility Complex Class I Recognition During T Cell Development in Vivo and By T Cell Receptor Stimulation of CD4+8+ Immature Thymocytes in Vitro. Eur J Immunol. 1996;26(10):2371-5. PubMed PMID: 8898947.
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TY - JOUR T1 - Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro. AU - Park,J H, AU - Hanke,T, AU - Hünig,T, PY - 1996/10/1/pubmed PY - 1996/10/1/medline PY - 1996/10/1/entrez SP - 2371 EP - 5 JF - European journal of immunology JO - Eur J Immunol VL - 26 IS - 10 N2 - A mouse monoclonal antibody (mAb) to the rat interleukin-2 receptor beta (IL-2R beta) chain was generated using IL-2R beta cDNA-transfected mouse L929 cells for immunization and differential screening. This antibody, called L316, detects a cell surface protein with an apparent molecular mass of about 80 kDa. In peripheral lymphoid organs of young adult rats, IL-2R beta expression is restricted to T and natural killer (NK) cells, and less than 10% of IL-2R beta+ cells co-express the IL-2R alpha chain. IL-2R beta was detected on all NKRP-1hi (NK-) and NKRP-1lo cells (T-lineage cells of unknown function), most peripheral gama delta T cells and on 30-40 % of CD8 and 10 % of CD4 alpha beta T cells. In the adult rat thymus, mAb L316 detects a small subset (about 1%) of predominantly IL-2R alpha- cells which express cell surface markers characteristic of mature T lymphocytes and contain a high proportion of CD4-8- and CD4-8+ alpha beta T cell receptor (TCR)+ thymocytes. TCR-V usage suggests that major histocompatibility complex (MHC) class I plays a more important role than MHC class II in the selection of these cells. On immature CD4+8+ rat thymocytes, IL-2R beta cell surface expression is readily induced by TCR stimulation in vitro, supporting the idea that in vivo, the IL-2R beta+ phenotype is the the result of TCR engagement during thymic selection. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/8898947/Identification_and_cellular_distribution_of_the_rat_interleukin_2_receptor_beta_chain:_induction_of_the_IL_2R_alpha__beta+_phenotype_by_major_histocompatibility_complex_class_I_recognition_during_T_cell_development_in_vivo_and_by_T_cell_receptor_stimulation_of_CD4+8+_immature_thymocytes_in_vitro_ L2 - https://doi.org/10.1002/eji.1830261015 DB - PRIME DP - Unbound Medicine ER -