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Modular elements of the MLC 1f/3f locus confer fiber-specific transcription regulation in transgenic mice.
Dev Genet. 1996; 19(2):157-62.DG

Abstract

The two proteins encoded by the fast alkali myosin light chain (MLC) 1f/3f locus are developmentally regulated, muscle specific, and expressed exclusively in fast-twitch fibers. Their expression is independently regulated by two separate promoters and a downstream enhancer. Previous studies showed a reporter gene directed by the rat MLC If promoter and MLC enhancer to exhibit correct skeletal muscle-specific expression in transgenic mice during development and to be preferentially expressed in fast-twitch Type IIB fibers [Donoghue et al., (1991) J. Cell B.ol. 115:423-434]. The MLC 3f promoter also directed muscle-specific expression of a CAT reporter gene in adult transgenic mice and showed little dependence upon the enhancer. Here, we show that the MLC 3f promoter also directs transgene expression in the fast-twitch fibers of adult skeletal muscle, but almost exclusively to fiber Types IIA and IIX. MLC 3f transgene expression occurs in only a subset of the fiber types that express the endogenous locus, indicating modular elements included in the transgene confer fiber-specific transcription regulation. MyoD protein was also found to be restricted to fiber Types IIA and IIX, providing evidence for its possible role in mediating fiber-specific gene expression.

Authors+Show Affiliations

Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129-2060, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8900048

Citation

Neville, C, et al. "Modular Elements of the MLC 1f/3f Locus Confer Fiber-specific Transcription Regulation in Transgenic Mice." Developmental Genetics, vol. 19, no. 2, 1996, pp. 157-62.
Neville C, Gonzales D, Houghton L, et al. Modular elements of the MLC 1f/3f locus confer fiber-specific transcription regulation in transgenic mice. Dev Genet. 1996;19(2):157-62.
Neville, C., Gonzales, D., Houghton, L., McGrew, M. J., & Rosenthal, N. (1996). Modular elements of the MLC 1f/3f locus confer fiber-specific transcription regulation in transgenic mice. Developmental Genetics, 19(2), 157-62.
Neville C, et al. Modular Elements of the MLC 1f/3f Locus Confer Fiber-specific Transcription Regulation in Transgenic Mice. Dev Genet. 1996;19(2):157-62. PubMed PMID: 8900048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modular elements of the MLC 1f/3f locus confer fiber-specific transcription regulation in transgenic mice. AU - Neville,C, AU - Gonzales,D, AU - Houghton,L, AU - McGrew,M J, AU - Rosenthal,N, PY - 1996/1/1/pubmed PY - 2000/6/20/medline PY - 1996/1/1/entrez SP - 157 EP - 62 JF - Developmental genetics JO - Dev Genet VL - 19 IS - 2 N2 - The two proteins encoded by the fast alkali myosin light chain (MLC) 1f/3f locus are developmentally regulated, muscle specific, and expressed exclusively in fast-twitch fibers. Their expression is independently regulated by two separate promoters and a downstream enhancer. Previous studies showed a reporter gene directed by the rat MLC If promoter and MLC enhancer to exhibit correct skeletal muscle-specific expression in transgenic mice during development and to be preferentially expressed in fast-twitch Type IIB fibers [Donoghue et al., (1991) J. Cell B.ol. 115:423-434]. The MLC 3f promoter also directed muscle-specific expression of a CAT reporter gene in adult transgenic mice and showed little dependence upon the enhancer. Here, we show that the MLC 3f promoter also directs transgene expression in the fast-twitch fibers of adult skeletal muscle, but almost exclusively to fiber Types IIA and IIX. MLC 3f transgene expression occurs in only a subset of the fiber types that express the endogenous locus, indicating modular elements included in the transgene confer fiber-specific transcription regulation. MyoD protein was also found to be restricted to fiber Types IIA and IIX, providing evidence for its possible role in mediating fiber-specific gene expression. SN - 0192-253X UR - https://www.unboundmedicine.com/medline/citation/8900048/Modular_elements_of_the_MLC_1f/3f_locus_confer_fiber_specific_transcription_regulation_in_transgenic_mice_ L2 - https://doi.org/10.1002/(SICI)1520-6408(1996)19:2<157::AID-DVG7>3.0.CO;2-8 DB - PRIME DP - Unbound Medicine ER -