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Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection.
Electrophoresis. 1996 Feb; 17(2):352-8.E

Abstract

A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of carbohydrate derivatized and determined by CE/LIF was 10 pmol contained in 100 microL of the final reaction mixture. The CE/LIF method provided a linear response for the carbohydrates tested (over 2-3 orders of magnitude), with a limit of detection of 100 amol. Separation efficiencies over 250,000 theoretical plates were obtained. The procedure was used to analyze a tear fluid sample to demonstrate the applicability of the method for the glucose determination in small volumes of biological samples.

Authors+Show Affiliations

Perez SA
Department of Chemistry, State University of New York at Buffalo 14260-3000,
Colón LA
No affiliation info available

MeSH

Dansyl CompoundsDisaccharidesElectrophoresis, CapillaryFluorescenceFluorescent DyesHumansHydrazinesLasersMolecular StructureMonosaccharidesTears

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8900942

Citation

Perez, S A., and L A. Colón. "Determination of Carbohydrates as Their Dansylhydrazine Derivatives By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Electrophoresis, vol. 17, no. 2, 1996, pp. 352-8.
Perez SA, Colón LA. Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 1996;17(2):352-8.
Perez, S. A., & Colón, L. A. (1996). Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 17(2), 352-8.
Perez SA, Colón LA. Determination of Carbohydrates as Their Dansylhydrazine Derivatives By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Electrophoresis. 1996;17(2):352-8. PubMed PMID: 8900942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of carbohydrates as their dansylhydrazine derivatives by capillary electrophoresis with laser-induced fluorescence detection. AU - Perez,S A, AU - Colón,L A, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 352 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 17 IS - 2 N2 - A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of carbohydrate derivatized and determined by CE/LIF was 10 pmol contained in 100 microL of the final reaction mixture. The CE/LIF method provided a linear response for the carbohydrates tested (over 2-3 orders of magnitude), with a limit of detection of 100 amol. Separation efficiencies over 250,000 theoretical plates were obtained. The procedure was used to analyze a tear fluid sample to demonstrate the applicability of the method for the glucose determination in small volumes of biological samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/8900942/Determination_of_carbohydrates_as_their_dansylhydrazine_derivatives_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -