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High performance capillary electrophoresis method to characterize heparin and heparan sulfate disaccharides.
Electrophoresis. 1996 Feb; 17(2):391-5.E

Abstract

A rapid, sensitive and accurate high-performance capillary electrophoresis method is described for the determination of the sulfation pattern of heparin and heparan sulfate disaccharides. The analysis, performed after enzymic degradation of the polysaccharides with heparinase and heparinases II and III in combination, yields highly UV-absorbing delta-disaccharides. The separation is performed with reversed polarity using 15 mM phosphate buffer, pH 3.50. This method is superior to others since all known 12 disaccharides carrying N-acetylated, N-sulfated or unsubstituent glucosamine can be separated in a single run of 15 min. At the highest sensitivity the analysis consumes only a few femtograms of glycosaminoglycan and allows a determination of delta-disaccharides at the attomole level.

Authors+Show Affiliations

Karamanos NK
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital F-42, Sweden.
Vanky P
No affiliation info available
Tzanakakis GN
No affiliation info available
Hjerpe A
No affiliation info available

MeSH

Carbohydrate SequenceDisaccharidesElectrophoresis, CapillaryHeparinHeparitin SulfateMolecular Sequence DataSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8900948

Citation

Karamanos, N K., et al. "High Performance Capillary Electrophoresis Method to Characterize Heparin and Heparan Sulfate Disaccharides." Electrophoresis, vol. 17, no. 2, 1996, pp. 391-5.
Karamanos NK, Vanky P, Tzanakakis GN, et al. High performance capillary electrophoresis method to characterize heparin and heparan sulfate disaccharides. Electrophoresis. 1996;17(2):391-5.
Karamanos, N. K., Vanky, P., Tzanakakis, G. N., & Hjerpe, A. (1996). High performance capillary electrophoresis method to characterize heparin and heparan sulfate disaccharides. Electrophoresis, 17(2), 391-5.
Karamanos NK, et al. High Performance Capillary Electrophoresis Method to Characterize Heparin and Heparan Sulfate Disaccharides. Electrophoresis. 1996;17(2):391-5. PubMed PMID: 8900948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High performance capillary electrophoresis method to characterize heparin and heparan sulfate disaccharides. AU - Karamanos,N K, AU - Vanky,P, AU - Tzanakakis,G N, AU - Hjerpe,A, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 391 EP - 5 JF - Electrophoresis JO - Electrophoresis VL - 17 IS - 2 N2 - A rapid, sensitive and accurate high-performance capillary electrophoresis method is described for the determination of the sulfation pattern of heparin and heparan sulfate disaccharides. The analysis, performed after enzymic degradation of the polysaccharides with heparinase and heparinases II and III in combination, yields highly UV-absorbing delta-disaccharides. The separation is performed with reversed polarity using 15 mM phosphate buffer, pH 3.50. This method is superior to others since all known 12 disaccharides carrying N-acetylated, N-sulfated or unsubstituent glucosamine can be separated in a single run of 15 min. At the highest sensitivity the analysis consumes only a few femtograms of glycosaminoglycan and allows a determination of delta-disaccharides at the attomole level. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/8900948/High_performance_capillary_electrophoresis_method_to_characterize_heparin_and_heparan_sulfate_disaccharides_ L2 - https://doi.org/10.1002/elps.1150170217 DB - PRIME DP - Unbound Medicine ER -
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