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Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice.
Biol Reprod 1996; 55(5):1063-8BR

Abstract

The effects of reactive oxygen species (ROS) on mouse sperm-egg fusion were determined. Sperm were treated with superoxide and hydrogen peroxide generated by addition of xanthine oxidase (XO: 10-200 mlU/ml) to hypoxanthine (HX: 1 mM). While XO at concentrations higher than 100 mlU/ml decreased the motility and lipid peroxidation of sperm, XO at less than 50 mlU/ml had no such effect. However, 20-50 mlU/ml XO significantly suppressed sperm fusion with zona-free eggs. Two ROS scavengers, superoxide dismutase and catalase, attenuated the inhibition of sperm-egg fusion by HX-XO. The sulfhydryl (SH) reductant, dithiothreitol, also reversed the inhibition. The sperm SH-rich fusion-related proteins were highly sensitive to ROS. These results suggest that ROS at low concentrations may inhibit sperm-egg fusion via oxidation of the SH-proteins in the sperm membrane, without causing loss of motility.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8902218

Citation

Mammoto, A, et al. "Reactive Oxygen Species Block Sperm-egg Fusion Via Oxidation of Sperm Sulfhydryl Proteins in Mice." Biology of Reproduction, vol. 55, no. 5, 1996, pp. 1063-8.
Mammoto A, Masumoto N, Tahara M, et al. Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biol Reprod. 1996;55(5):1063-8.
Mammoto, A., Masumoto, N., Tahara, M., Ikebuchi, Y., Ohmichi, M., Tasaka, K., & Miyake, A. (1996). Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biology of Reproduction, 55(5), pp. 1063-8.
Mammoto A, et al. Reactive Oxygen Species Block Sperm-egg Fusion Via Oxidation of Sperm Sulfhydryl Proteins in Mice. Biol Reprod. 1996;55(5):1063-8. PubMed PMID: 8902218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. AU - Mammoto,A, AU - Masumoto,N, AU - Tahara,M, AU - Ikebuchi,Y, AU - Ohmichi,M, AU - Tasaka,K, AU - Miyake,A, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 1063 EP - 8 JF - Biology of reproduction JO - Biol. Reprod. VL - 55 IS - 5 N2 - The effects of reactive oxygen species (ROS) on mouse sperm-egg fusion were determined. Sperm were treated with superoxide and hydrogen peroxide generated by addition of xanthine oxidase (XO: 10-200 mlU/ml) to hypoxanthine (HX: 1 mM). While XO at concentrations higher than 100 mlU/ml decreased the motility and lipid peroxidation of sperm, XO at less than 50 mlU/ml had no such effect. However, 20-50 mlU/ml XO significantly suppressed sperm fusion with zona-free eggs. Two ROS scavengers, superoxide dismutase and catalase, attenuated the inhibition of sperm-egg fusion by HX-XO. The sulfhydryl (SH) reductant, dithiothreitol, also reversed the inhibition. The sperm SH-rich fusion-related proteins were highly sensitive to ROS. These results suggest that ROS at low concentrations may inhibit sperm-egg fusion via oxidation of the SH-proteins in the sperm membrane, without causing loss of motility. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/8902218/Reactive_oxygen_species_block_sperm_egg_fusion_via_oxidation_of_sperm_sulfhydryl_proteins_in_mice_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod55.5.1063 DB - PRIME DP - Unbound Medicine ER -