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[Experimental techniques for developing new drugs acting on dementia (11)--Experimental methods on glutamate neurotoxicity].
Nihon Shinkei Seishin Yakurigaku Zasshi. 1996 Jun; 16(3):91-6.NS

Abstract

Glutamate-induced neurotoxicity was examined in cultured rat cortical cells. Primary cultures were obtained from the cerebral cortex of fetal rats (17-19 days of gestation). Single cells dissociated from the cerebral cortex were plated on plastic coverslips placed in 35- or 60-mm culture dishes. Cultures were incubated in Eagle's minimal essential medium supplemented with 10% fetal calf serum or 10% horse serum at 37 degrees C in a humidified 5% CO2 atmosphere for 10-14 days. The neurotoxicity induced by glutamate was quantified by trypan blue exclusion. The viability of cultures was markedly reduced by a 10-min exposure to glutamate followed by incubation with glutamate-free medium for 1-24 hr. Glutamate neurotoxicity was prevented by the N-methyl-D-aspartate (NMDA) receptor antagonists, MK-801, 3-[(+/-)-2-carboxypiperazin-4-yl] propyl-1-phosphoric acid (CPP), ifenprodil and 7-Cl-kinurenate. Glutamate neurotoxicity was augmented by phorbol dibutyrate, that activates protein kinase C (PKC), but reduced by H-7, that inhibits PKC. These results suggest that PKC plays an important role in NMDA receptor-mediated glutamate neurotoxicity in the cerebral cortex.

Authors+Show Affiliations

Akaike A
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Maeda T
No affiliation info available
Tamura Y
No affiliation info available

MeSH

AnimalsCell SurvivalCells, CulturedCerebral CortexDizocilpine MaleateGlutamic AcidPhorbol EstersProtein Kinase CRatsReceptors, N-Methyl-D-Aspartate

Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

8905796

Citation

Akaike, A, et al. "[Experimental Techniques for Developing New Drugs Acting On Dementia (11)--Experimental Methods On Glutamate Neurotoxicity]." Nihon Shinkei Seishin Yakurigaku Zasshi = Japanese Journal of Psychopharmacology, vol. 16, no. 3, 1996, pp. 91-6.
Akaike A, Maeda T, Tamura Y. [Experimental techniques for developing new drugs acting on dementia (11)--Experimental methods on glutamate neurotoxicity]. Nihon Shinkei Seishin Yakurigaku Zasshi. 1996;16(3):91-6.
Akaike, A., Maeda, T., & Tamura, Y. (1996). [Experimental techniques for developing new drugs acting on dementia (11)--Experimental methods on glutamate neurotoxicity]. Nihon Shinkei Seishin Yakurigaku Zasshi = Japanese Journal of Psychopharmacology, 16(3), 91-6.
Akaike A, Maeda T, Tamura Y. [Experimental Techniques for Developing New Drugs Acting On Dementia (11)--Experimental Methods On Glutamate Neurotoxicity]. Nihon Shinkei Seishin Yakurigaku Zasshi. 1996;16(3):91-6. PubMed PMID: 8905796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Experimental techniques for developing new drugs acting on dementia (11)--Experimental methods on glutamate neurotoxicity]. AU - Akaike,A, AU - Maeda,T, AU - Tamura,Y, PY - 1996/6/1/pubmed PY - 1996/6/1/medline PY - 1996/6/1/entrez SP - 91 EP - 6 JF - Nihon shinkei seishin yakurigaku zasshi = Japanese journal of psychopharmacology JO - Nihon Shinkei Seishin Yakurigaku Zasshi VL - 16 IS - 3 N2 - Glutamate-induced neurotoxicity was examined in cultured rat cortical cells. Primary cultures were obtained from the cerebral cortex of fetal rats (17-19 days of gestation). Single cells dissociated from the cerebral cortex were plated on plastic coverslips placed in 35- or 60-mm culture dishes. Cultures were incubated in Eagle's minimal essential medium supplemented with 10% fetal calf serum or 10% horse serum at 37 degrees C in a humidified 5% CO2 atmosphere for 10-14 days. The neurotoxicity induced by glutamate was quantified by trypan blue exclusion. The viability of cultures was markedly reduced by a 10-min exposure to glutamate followed by incubation with glutamate-free medium for 1-24 hr. Glutamate neurotoxicity was prevented by the N-methyl-D-aspartate (NMDA) receptor antagonists, MK-801, 3-[(+/-)-2-carboxypiperazin-4-yl] propyl-1-phosphoric acid (CPP), ifenprodil and 7-Cl-kinurenate. Glutamate neurotoxicity was augmented by phorbol dibutyrate, that activates protein kinase C (PKC), but reduced by H-7, that inhibits PKC. These results suggest that PKC plays an important role in NMDA receptor-mediated glutamate neurotoxicity in the cerebral cortex. SN - 1340-2544 UR - https://www.unboundmedicine.com/medline/citation/8905796/[Experimental_techniques_for_developing_new_drugs_acting_on_dementia__11___Experimental_methods_on_glutamate_neurotoxicity]_ DB - PRIME DP - Unbound Medicine ER -