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Intracellular CD22 rapidly moves to the cell surface in a tyrosine kinase-dependent manner following antigen receptor stimulation.
J Immunol. 1996 Nov 15; 157(10):4390-8.JI

Abstract

CD22 is a key accessory molecule for Ag receptor signaling in B cells that becomes tyrosine phosphorylated in the signaling process. CD22 associates with sIg and strongly amplifies sIg-induced signals. During B cell development, CD22 is initially expressed intracellularly, with surface expression appearing with IgD expression. We used confocal laser-scanning microscopy and flow cytometry to analyze CD22 translocation responses to signaling events. Cross-linking surface IgM (sIgM) induced rapid movement of CD22 to the cell surface in both Ramos and Daudi B cells, with a 50 to 100% increase in surface expression observed within 5 min of stimulation. The increase in CD22 surface expression was specific in that CD19 expression was not affected. Both cell surface and intracellular CD22 were directed toward the site of sIgM stimulation. Treatment with the phosphotyrosine phosphatase inhibitor bis(maltolato)oxovanadium(IV) also increased CD22 surface expression. The tyrosine kinase inhibitor tyrphostin A10 inhibited CD22 movement at concentrations that inhibited tyrosine phosphorylation of CD22 and other cellular proteins. In contrast to the B cell lines, mature peripheral blood B cells contained very little intracellular CD22 and showed no significant increase in surface expression following sIgM stimulation. The rapid directed movement of intracellular CD22 provides a new mechanism to dynamically regulate Ag receptor signaling, as well as CD22-mediated adhesion.

Authors+Show Affiliations

Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8906814

Citation

Sherbina, N V., et al. "Intracellular CD22 Rapidly Moves to the Cell Surface in a Tyrosine Kinase-dependent Manner Following Antigen Receptor Stimulation." Journal of Immunology (Baltimore, Md. : 1950), vol. 157, no. 10, 1996, pp. 4390-8.
Sherbina NV, Linsley PS, Myrdal S, et al. Intracellular CD22 rapidly moves to the cell surface in a tyrosine kinase-dependent manner following antigen receptor stimulation. J Immunol. 1996;157(10):4390-8.
Sherbina, N. V., Linsley, P. S., Myrdal, S., Grosmaire, L. S., Ledbetter, J. A., & Schieven, G. L. (1996). Intracellular CD22 rapidly moves to the cell surface in a tyrosine kinase-dependent manner following antigen receptor stimulation. Journal of Immunology (Baltimore, Md. : 1950), 157(10), 4390-8.
Sherbina NV, et al. Intracellular CD22 Rapidly Moves to the Cell Surface in a Tyrosine Kinase-dependent Manner Following Antigen Receptor Stimulation. J Immunol. 1996 Nov 15;157(10):4390-8. PubMed PMID: 8906814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intracellular CD22 rapidly moves to the cell surface in a tyrosine kinase-dependent manner following antigen receptor stimulation. AU - Sherbina,N V, AU - Linsley,P S, AU - Myrdal,S, AU - Grosmaire,L S, AU - Ledbetter,J A, AU - Schieven,G L, PY - 1996/11/15/pubmed PY - 1996/11/15/medline PY - 1996/11/15/entrez SP - 4390 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 157 IS - 10 N2 - CD22 is a key accessory molecule for Ag receptor signaling in B cells that becomes tyrosine phosphorylated in the signaling process. CD22 associates with sIg and strongly amplifies sIg-induced signals. During B cell development, CD22 is initially expressed intracellularly, with surface expression appearing with IgD expression. We used confocal laser-scanning microscopy and flow cytometry to analyze CD22 translocation responses to signaling events. Cross-linking surface IgM (sIgM) induced rapid movement of CD22 to the cell surface in both Ramos and Daudi B cells, with a 50 to 100% increase in surface expression observed within 5 min of stimulation. The increase in CD22 surface expression was specific in that CD19 expression was not affected. Both cell surface and intracellular CD22 were directed toward the site of sIgM stimulation. Treatment with the phosphotyrosine phosphatase inhibitor bis(maltolato)oxovanadium(IV) also increased CD22 surface expression. The tyrosine kinase inhibitor tyrphostin A10 inhibited CD22 movement at concentrations that inhibited tyrosine phosphorylation of CD22 and other cellular proteins. In contrast to the B cell lines, mature peripheral blood B cells contained very little intracellular CD22 and showed no significant increase in surface expression following sIgM stimulation. The rapid directed movement of intracellular CD22 provides a new mechanism to dynamically regulate Ag receptor signaling, as well as CD22-mediated adhesion. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8906814/Intracellular_CD22_rapidly_moves_to_the_cell_surface_in_a_tyrosine_kinase_dependent_manner_following_antigen_receptor_stimulation_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8906814 DB - PRIME DP - Unbound Medicine ER -