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Immunoglobulin G response against 10-kDa and 65-kDa heat-shock proteins in leprosy patients and their household contacts.
FEMS Immunol Med Microbiol. 1996 Oct; 15(4):189-98.FI

Abstract

We measured antibody responses to recombinant Mycobacterium leprae 65-kDa (rML65) and 10-kDa (rML10) by indirect ELISA in sera from leprosy patients, household contacts and healthy controls in a leprosy-endemic area in the north east of Argentina. Serum antibody levels to those antigens were correlated with IgM anti-phenolic glycolipid I (PGL-I) levels, with bacterial index and the period of time under chemotherapy. Bacterial index positive (BI+) patients showed higher mean values when compared with BI negatives (BI-). Among lepromatous patients a positive correlation was observed between IgG antibody responses to both recombinant antigens and IgM antibody response to PGL-I. Anti-rML10 test detected a higher percentage of positives/total than anti-rML65 in all leprosy groups and healthy contacts. Bacterial load, leprosy clinical form and the time under chemotherapy were factors which could influence levels of the antibody response. The contribution of these antibody studies for a precise and early diagnosis in leprosy is discussed.

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Authors+Show Affiliations

Rojas RE
CINIBA (Centro de Investigaciones Inmunológicas Básicas y Aplicadas), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
Segal-Eiras A
No affiliation info available

MeSH

AdultAgedAntibodies, BacterialAntibodies, MonoclonalAntigens, BacterialArgentinaBacterial ProteinsChaperonin 10Chaperonin 60ChaperoninsDrug Therapy, CombinationEnzyme-Linked Immunosorbent AssayGlycolipidsHumansImmunoglobulin GImmunoglobulin MLeprosyMiddle AgedMycobacterium lepraeRecombinant ProteinsSeroepidemiologic StudiesTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8908480

Citation

Rojas, R E., and A Segal-Eiras. "Immunoglobulin G Response Against 10-kDa and 65-kDa Heat-shock Proteins in Leprosy Patients and Their Household Contacts." FEMS Immunology and Medical Microbiology, vol. 15, no. 4, 1996, pp. 189-98.
Rojas RE, Segal-Eiras A. Immunoglobulin G response against 10-kDa and 65-kDa heat-shock proteins in leprosy patients and their household contacts. FEMS Immunol Med Microbiol. 1996;15(4):189-98.
Rojas, R. E., & Segal-Eiras, A. (1996). Immunoglobulin G response against 10-kDa and 65-kDa heat-shock proteins in leprosy patients and their household contacts. FEMS Immunology and Medical Microbiology, 15(4), 189-98.
Rojas RE, Segal-Eiras A. Immunoglobulin G Response Against 10-kDa and 65-kDa Heat-shock Proteins in Leprosy Patients and Their Household Contacts. FEMS Immunol Med Microbiol. 1996;15(4):189-98. PubMed PMID: 8908480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoglobulin G response against 10-kDa and 65-kDa heat-shock proteins in leprosy patients and their household contacts. AU - Rojas,R E, AU - Segal-Eiras,A, PY - 1996/10/1/pubmed PY - 1996/10/1/medline PY - 1996/10/1/entrez SP - 189 EP - 98 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 15 IS - 4 N2 - We measured antibody responses to recombinant Mycobacterium leprae 65-kDa (rML65) and 10-kDa (rML10) by indirect ELISA in sera from leprosy patients, household contacts and healthy controls in a leprosy-endemic area in the north east of Argentina. Serum antibody levels to those antigens were correlated with IgM anti-phenolic glycolipid I (PGL-I) levels, with bacterial index and the period of time under chemotherapy. Bacterial index positive (BI+) patients showed higher mean values when compared with BI negatives (BI-). Among lepromatous patients a positive correlation was observed between IgG antibody responses to both recombinant antigens and IgM antibody response to PGL-I. Anti-rML10 test detected a higher percentage of positives/total than anti-rML65 in all leprosy groups and healthy contacts. Bacterial load, leprosy clinical form and the time under chemotherapy were factors which could influence levels of the antibody response. The contribution of these antibody studies for a precise and early diagnosis in leprosy is discussed. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/8908480/Immunoglobulin_G_response_against_10_kDa_and_65_kDa_heat_shock_proteins_in_leprosy_patients_and_their_household_contacts_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=1996&volume=15&issue=4&spage=189 DB - PRIME DP - Unbound Medicine ER -