Tags

Type your tag names separated by a space and hit enter

Expression ratio of hepatocyte nuclear factor-1 to variant hepatocyte nuclear factor-1 in differentiation of hepatocellular carcinoma and hepatoblastoma.
J Hepatol. 1996 Oct; 25(4):445-53.JH

Abstract

BACKGROUND/AIMS

Liver-specific protein genes have multiple cis-/trans-acting elements, but those accountable for hepatocytic differentiation are unclear. An AT-rich core sequence (AT motif) is essential as a cis-acting element for the hepatic transcription. Homologous proteins hepatocyte nuclear factor-1 (HNF-1) and variant HNF-1 (vHNF-1) bind to this motif. The ratio of HNF-1 to vHNF-1 mRNA was examined in various liver tissues with respect to their differentiation.

METHODS

The competitive reverse transcriptional polymerase chain reaction was employed to amplify HNF-1 and vHNF-1 mRNA simultaneously and to examine their expression ratio in total RNA extracted from frozen liver tissues of 37 patients with hepatocellular carcinoma, five patients with hepatoblastoma, and 15 non-neoplastic liver tissues.

RESULTS

The ratio of HNF-1 to vHNF-1 mRNA was higher in well-differentiated cases than in poorly-differentiated and undifferentiated cases, except that one poorly-differentiated hepatoblastoma displayed a high ratio. Non-neoplastic liver tissues had low ratios similar to poorly-differentiated hepatocellular carcinoma, the reason for which remained unknown. However, chronic hepatitis and liver cirrhosis cases also demonstrated low ratios, and hence degenerative changes themselves displayed no obvious influence on such ratios. Thus, the gene expression of HNF-1 and vHNF-1 seemed to be differentially regulated in neoplastic and non-neoplastic hepatocytes.

CONCLUSIONS

These results suggested that the ratio of HNF-1 to vHNF-1 mRNA correlated with histological differentiation of HCC and hepatoblastoma.

Authors+Show Affiliations

First Department of Pathology, Kobe University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8912143

Citation

Ninomiya, T, et al. "Expression Ratio of Hepatocyte Nuclear Factor-1 to Variant Hepatocyte Nuclear Factor-1 in Differentiation of Hepatocellular Carcinoma and Hepatoblastoma." Journal of Hepatology, vol. 25, no. 4, 1996, pp. 445-53.
Ninomiya T, Hayashi Y, Saijoh K, et al. Expression ratio of hepatocyte nuclear factor-1 to variant hepatocyte nuclear factor-1 in differentiation of hepatocellular carcinoma and hepatoblastoma. J Hepatol. 1996;25(4):445-53.
Ninomiya, T., Hayashi, Y., Saijoh, K., Ohta, K., Yoon, S., Nakabayashi, H., Tamaoki, T., Kasuga, M., & Itoh, H. (1996). Expression ratio of hepatocyte nuclear factor-1 to variant hepatocyte nuclear factor-1 in differentiation of hepatocellular carcinoma and hepatoblastoma. Journal of Hepatology, 25(4), 445-53.
Ninomiya T, et al. Expression Ratio of Hepatocyte Nuclear Factor-1 to Variant Hepatocyte Nuclear Factor-1 in Differentiation of Hepatocellular Carcinoma and Hepatoblastoma. J Hepatol. 1996;25(4):445-53. PubMed PMID: 8912143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression ratio of hepatocyte nuclear factor-1 to variant hepatocyte nuclear factor-1 in differentiation of hepatocellular carcinoma and hepatoblastoma. AU - Ninomiya,T, AU - Hayashi,Y, AU - Saijoh,K, AU - Ohta,K, AU - Yoon,S, AU - Nakabayashi,H, AU - Tamaoki,T, AU - Kasuga,M, AU - Itoh,H, PY - 1996/10/1/pubmed PY - 1996/10/1/medline PY - 1996/10/1/entrez SP - 445 EP - 53 JF - Journal of hepatology JO - J Hepatol VL - 25 IS - 4 N2 - BACKGROUND/AIMS: Liver-specific protein genes have multiple cis-/trans-acting elements, but those accountable for hepatocytic differentiation are unclear. An AT-rich core sequence (AT motif) is essential as a cis-acting element for the hepatic transcription. Homologous proteins hepatocyte nuclear factor-1 (HNF-1) and variant HNF-1 (vHNF-1) bind to this motif. The ratio of HNF-1 to vHNF-1 mRNA was examined in various liver tissues with respect to their differentiation. METHODS: The competitive reverse transcriptional polymerase chain reaction was employed to amplify HNF-1 and vHNF-1 mRNA simultaneously and to examine their expression ratio in total RNA extracted from frozen liver tissues of 37 patients with hepatocellular carcinoma, five patients with hepatoblastoma, and 15 non-neoplastic liver tissues. RESULTS: The ratio of HNF-1 to vHNF-1 mRNA was higher in well-differentiated cases than in poorly-differentiated and undifferentiated cases, except that one poorly-differentiated hepatoblastoma displayed a high ratio. Non-neoplastic liver tissues had low ratios similar to poorly-differentiated hepatocellular carcinoma, the reason for which remained unknown. However, chronic hepatitis and liver cirrhosis cases also demonstrated low ratios, and hence degenerative changes themselves displayed no obvious influence on such ratios. Thus, the gene expression of HNF-1 and vHNF-1 seemed to be differentially regulated in neoplastic and non-neoplastic hepatocytes. CONCLUSIONS: These results suggested that the ratio of HNF-1 to vHNF-1 mRNA correlated with histological differentiation of HCC and hepatoblastoma. SN - 0168-8278 UR - https://www.unboundmedicine.com/medline/citation/8912143/Expression_ratio_of_hepatocyte_nuclear_factor_1_to_variant_hepatocyte_nuclear_factor_1_in_differentiation_of_hepatocellular_carcinoma_and_hepatoblastoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-8278(96)80203-0 DB - PRIME DP - Unbound Medicine ER -