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Intestinal adaptation following massive small bowel resection in the mouse.
J Am Coll Surg. 1996 Nov; 183(5):441-9.JA

Abstract

BACKGROUND

Transgenic mice represent powerful tools for studying the role of genes and their expression under multiple conditions, and they may provide a unique model for studies of intestinal adaptation after massive small bowel resection (SBR). This study characterized a successful model for SBR and intestinal adaptation in the mouse.

STUDY DESIGN

Sham operation (bowel transection with reanastomosis) or SBR was performed on male C57BL/6 mice. A solid or liquid diet, various sizes of monofilament suture for the anastomosis, and resection of 50 or 75 percent of the proximal small intestine were studied. In other studies, intestinal adaptation was characterized as changes in intestinal wet weight, DNA, protein, villus height, crypt depth, and crypt cell proliferation rates at 12 hours, 24 hours, three days, and one, two, and four weeks after 50 percent SBR.

RESULTS

Survival was significantly improved with a liquid diet (8 percent compared with 88 percent; p < .001) and modestly improved by using the smallest suture for anastomosis (60 percent for 7-0 compared with 88 percent for 9-0; p = not significant). Mice did not tolerate more than 50 percent SBR (16 percent survival rate for 75 percent SBR compared with 85 percent survival rate for 50 percent SBR; p < .01). Small bowel resection augmented ileal wet weight, DNA and protein content, villus height, crypt depth, and crypt-cell proliferation rates.

CONCLUSIONS

Provision of a liquid diet, using a small suture for anastomosis, and resection of no more than 50 percent of the proximal small intestine are important for survival. This model will permit researchers using transgenic mice to better understand critical genes during intestinal adaptation after SBR.

Authors+Show Affiliations

Division of Pediatric Surgery, Children's Hospital Medical Center, University of Cincinnati College of Medicine, OH 45229-3039, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8912612

Citation

Helmrath, M A., et al. "Intestinal Adaptation Following Massive Small Bowel Resection in the Mouse." Journal of the American College of Surgeons, vol. 183, no. 5, 1996, pp. 441-9.
Helmrath MA, VanderKolk WE, Can G, et al. Intestinal adaptation following massive small bowel resection in the mouse. J Am Coll Surg. 1996;183(5):441-9.
Helmrath, M. A., VanderKolk, W. E., Can, G., Erwin, C. R., & Warner, B. W. (1996). Intestinal adaptation following massive small bowel resection in the mouse. Journal of the American College of Surgeons, 183(5), 441-9.
Helmrath MA, et al. Intestinal Adaptation Following Massive Small Bowel Resection in the Mouse. J Am Coll Surg. 1996;183(5):441-9. PubMed PMID: 8912612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intestinal adaptation following massive small bowel resection in the mouse. AU - Helmrath,M A, AU - VanderKolk,W E, AU - Can,G, AU - Erwin,C R, AU - Warner,B W, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 441 EP - 9 JF - Journal of the American College of Surgeons JO - J Am Coll Surg VL - 183 IS - 5 N2 - BACKGROUND: Transgenic mice represent powerful tools for studying the role of genes and their expression under multiple conditions, and they may provide a unique model for studies of intestinal adaptation after massive small bowel resection (SBR). This study characterized a successful model for SBR and intestinal adaptation in the mouse. STUDY DESIGN: Sham operation (bowel transection with reanastomosis) or SBR was performed on male C57BL/6 mice. A solid or liquid diet, various sizes of monofilament suture for the anastomosis, and resection of 50 or 75 percent of the proximal small intestine were studied. In other studies, intestinal adaptation was characterized as changes in intestinal wet weight, DNA, protein, villus height, crypt depth, and crypt cell proliferation rates at 12 hours, 24 hours, three days, and one, two, and four weeks after 50 percent SBR. RESULTS: Survival was significantly improved with a liquid diet (8 percent compared with 88 percent; p < .001) and modestly improved by using the smallest suture for anastomosis (60 percent for 7-0 compared with 88 percent for 9-0; p = not significant). Mice did not tolerate more than 50 percent SBR (16 percent survival rate for 75 percent SBR compared with 85 percent survival rate for 50 percent SBR; p < .01). Small bowel resection augmented ileal wet weight, DNA and protein content, villus height, crypt depth, and crypt-cell proliferation rates. CONCLUSIONS: Provision of a liquid diet, using a small suture for anastomosis, and resection of no more than 50 percent of the proximal small intestine are important for survival. This model will permit researchers using transgenic mice to better understand critical genes during intestinal adaptation after SBR. SN - 1072-7515 UR - https://www.unboundmedicine.com/medline/citation/8912612/Intestinal_adaptation_following_massive_small_bowel_resection_in_the_mouse_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:8912612 DB - PRIME DP - Unbound Medicine ER -