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Tissue-specific splicing of two mutually exclusive exons of the chicken beta-tropomyosin pre-mRNA: positive and negative regulations.
Biochimie. 1996; 78(6):457-65.B

Abstract

Alternative splicing of premessenger RNA (pre-mRNA) is a widespread process used in higher eucaryotes to regulate gene expression. A single primary transcript can generate multiple proteins with distinct functions in a tissue- and/or developmental-specific manner. A central question in alternative splicing concerns the selection of splice sites in different cell environments. In this review, we present our results on the alternative splicing of the chicken beta-tropomyosin gene which provides an interesting model for understanding mechanisms involved in splice site selection. The beta-tropomyosin gene contains in its central portion a pair of exons (6A and 6B) that are used mutually exclusively in a tissue and developmental stage-specific manner. Exon 6A is present in mRNA of non-muscle and smooth muscle tissues while exon 6B is only present in mRNA of skeletal muscle. Regulation of both exons is necessary to ensure specific expression of beta-tropomyosin gene in non-muscle cells. Several cis-acting elements involved in the repression of exon 6B and activation of exon 6A have been identified. In addition, we show that the tissue-specific choice of exon 6A is mediated through interaction with a specific class of splicing factors, the SR proteins. In the last part of this review we will focus on possible mechanisms needed to switch to exon 6B selection in skeletal muscle tissue. We propose that tissue-specific choice of exon 6B involves down regulation of exon 6A and activation of exon 6B. A G-rich enhancer sequence downstream of exon 6B has been defined that is needed for efficient recognition of the exon 6B 5' splice site. Moreover, we suggest that alteration of the ratio between proteins of the SR family contributes to tissue-specific splice site selection.

Authors+Show Affiliations

Centre de Génétique Moléculaire, CNRS, Laboratoire Propre Associé à l'Université Pierre-et-Marie-Curie, Gif-sur-Yvette, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

8915535

Citation

Gallego, M E., et al. "Tissue-specific Splicing of Two Mutually Exclusive Exons of the Chicken Beta-tropomyosin pre-mRNA: Positive and Negative Regulations." Biochimie, vol. 78, no. 6, 1996, pp. 457-65.
Gallego ME, Sirand-Pugnet P, Durosay P, et al. Tissue-specific splicing of two mutually exclusive exons of the chicken beta-tropomyosin pre-mRNA: positive and negative regulations. Biochimie. 1996;78(6):457-65.
Gallego, M. E., Sirand-Pugnet, P., Durosay, P., Clouet d'Orval, B., d'Aubenton-Carafa, Y., Brody, E., Expert-Bezançon, A., & Marie, J. (1996). Tissue-specific splicing of two mutually exclusive exons of the chicken beta-tropomyosin pre-mRNA: positive and negative regulations. Biochimie, 78(6), 457-65.
Gallego ME, et al. Tissue-specific Splicing of Two Mutually Exclusive Exons of the Chicken Beta-tropomyosin pre-mRNA: Positive and Negative Regulations. Biochimie. 1996;78(6):457-65. PubMed PMID: 8915535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-specific splicing of two mutually exclusive exons of the chicken beta-tropomyosin pre-mRNA: positive and negative regulations. AU - Gallego,M E, AU - Sirand-Pugnet,P, AU - Durosay,P, AU - Clouet d'Orval,B, AU - d'Aubenton-Carafa,Y, AU - Brody,E, AU - Expert-Bezançon,A, AU - Marie,J, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 457 EP - 65 JF - Biochimie JO - Biochimie VL - 78 IS - 6 N2 - Alternative splicing of premessenger RNA (pre-mRNA) is a widespread process used in higher eucaryotes to regulate gene expression. A single primary transcript can generate multiple proteins with distinct functions in a tissue- and/or developmental-specific manner. A central question in alternative splicing concerns the selection of splice sites in different cell environments. In this review, we present our results on the alternative splicing of the chicken beta-tropomyosin gene which provides an interesting model for understanding mechanisms involved in splice site selection. The beta-tropomyosin gene contains in its central portion a pair of exons (6A and 6B) that are used mutually exclusively in a tissue and developmental stage-specific manner. Exon 6A is present in mRNA of non-muscle and smooth muscle tissues while exon 6B is only present in mRNA of skeletal muscle. Regulation of both exons is necessary to ensure specific expression of beta-tropomyosin gene in non-muscle cells. Several cis-acting elements involved in the repression of exon 6B and activation of exon 6A have been identified. In addition, we show that the tissue-specific choice of exon 6A is mediated through interaction with a specific class of splicing factors, the SR proteins. In the last part of this review we will focus on possible mechanisms needed to switch to exon 6B selection in skeletal muscle tissue. We propose that tissue-specific choice of exon 6B involves down regulation of exon 6A and activation of exon 6B. A G-rich enhancer sequence downstream of exon 6B has been defined that is needed for efficient recognition of the exon 6B 5' splice site. Moreover, we suggest that alteration of the ratio between proteins of the SR family contributes to tissue-specific splice site selection. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/8915535/Tissue_specific_splicing_of_two_mutually_exclusive_exons_of_the_chicken_beta_tropomyosin_pre_mRNA:_positive_and_negative_regulations_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0300-9084(96)84752-3 DB - PRIME DP - Unbound Medicine ER -