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Ultrastructural localization of Shaker-related potassium channel subunits and synapse-associated protein 90 to septate-like junctions in rat cerebellar Pinceaux.
Brain Res Mol Brain Res. 1996 Nov; 42(1):51-61.BR

Abstract

The Pinceau is a paintbrush-like network of cerebellar basket cell axon branchlets embracing the initial segment of the Purkinje cell axon. Its electrical activity contributes to the control of the cerebellar cortical output through the Purkinje cell axon by generating an inhibitory field effect. In addition to the structural features of the Pinceau, its repertoire of voltage-gated ion channels is likely to be an important aspect of this function. Therefore, we investigated the fine structural distribution of voltage-activated potassium (Kv1.1, Kv1.2, Kv3.4) and sodium channel proteins in the Pinceau. The ultrastructural localization of potassium channel subunits was compared to the distribution of synapse-associated protein 90 (SAP90), a protein capable to induce in vitro clustering of Kv1 proteins. With an improved preembedding technique including ultrasmall gold particles, silver enhancement and gold toning, we could show that antibodies recognizing Kv1.1, Kv1.2 and SAP90 are predominantly localized to septate-like junctions, which connect the basket cell axonal branchlets. Kv3.4 immunoreactivity is not concentrated in junctional regions but uniformly distributed over the Pinceau and the pericellular basket surrounding the Purkinje cell soma. In contrast, voltage-activated sodium channels were not detected in the Pinceau, but localized to the Purkinje cell axon initial segment. The results suggest that Kv1.1 and Kv1.2 form heterooligomeric delayed rectifier type Kv channels, being colocalized to septate-like junctions by interaction with SAP90.

Authors+Show Affiliations

Zentrum für Molekulare Neurobiologie, Universität Hamburg, Germany. laube@plexus.uke.uni-hamburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8915580

Citation

Laube, G, et al. "Ultrastructural Localization of Shaker-related Potassium Channel Subunits and Synapse-associated Protein 90 to Septate-like Junctions in Rat Cerebellar Pinceaux." Brain Research. Molecular Brain Research, vol. 42, no. 1, 1996, pp. 51-61.
Laube G, Röper J, Pitt JC, et al. Ultrastructural localization of Shaker-related potassium channel subunits and synapse-associated protein 90 to septate-like junctions in rat cerebellar Pinceaux. Brain Res Mol Brain Res. 1996;42(1):51-61.
Laube, G., Röper, J., Pitt, J. C., Sewing, S., Kistner, U., Garner, C. C., Pongs, O., & Veh, R. W. (1996). Ultrastructural localization of Shaker-related potassium channel subunits and synapse-associated protein 90 to septate-like junctions in rat cerebellar Pinceaux. Brain Research. Molecular Brain Research, 42(1), 51-61.
Laube G, et al. Ultrastructural Localization of Shaker-related Potassium Channel Subunits and Synapse-associated Protein 90 to Septate-like Junctions in Rat Cerebellar Pinceaux. Brain Res Mol Brain Res. 1996;42(1):51-61. PubMed PMID: 8915580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural localization of Shaker-related potassium channel subunits and synapse-associated protein 90 to septate-like junctions in rat cerebellar Pinceaux. AU - Laube,G, AU - Röper,J, AU - Pitt,J C, AU - Sewing,S, AU - Kistner,U, AU - Garner,C C, AU - Pongs,O, AU - Veh,R W, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 51 EP - 61 JF - Brain research. Molecular brain research JO - Brain Res Mol Brain Res VL - 42 IS - 1 N2 - The Pinceau is a paintbrush-like network of cerebellar basket cell axon branchlets embracing the initial segment of the Purkinje cell axon. Its electrical activity contributes to the control of the cerebellar cortical output through the Purkinje cell axon by generating an inhibitory field effect. In addition to the structural features of the Pinceau, its repertoire of voltage-gated ion channels is likely to be an important aspect of this function. Therefore, we investigated the fine structural distribution of voltage-activated potassium (Kv1.1, Kv1.2, Kv3.4) and sodium channel proteins in the Pinceau. The ultrastructural localization of potassium channel subunits was compared to the distribution of synapse-associated protein 90 (SAP90), a protein capable to induce in vitro clustering of Kv1 proteins. With an improved preembedding technique including ultrasmall gold particles, silver enhancement and gold toning, we could show that antibodies recognizing Kv1.1, Kv1.2 and SAP90 are predominantly localized to septate-like junctions, which connect the basket cell axonal branchlets. Kv3.4 immunoreactivity is not concentrated in junctional regions but uniformly distributed over the Pinceau and the pericellular basket surrounding the Purkinje cell soma. In contrast, voltage-activated sodium channels were not detected in the Pinceau, but localized to the Purkinje cell axon initial segment. The results suggest that Kv1.1 and Kv1.2 form heterooligomeric delayed rectifier type Kv channels, being colocalized to septate-like junctions by interaction with SAP90. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/8915580/Ultrastructural_localization_of_Shaker_related_potassium_channel_subunits_and_synapse_associated_protein_90_to_septate_like_junctions_in_rat_cerebellar_Pinceaux_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169328X96001209 DB - PRIME DP - Unbound Medicine ER -