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Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis.
Proc Natl Acad Sci U S A. 1996 Nov 12; 93(23):12867-72.PN

Abstract

Ro09-0198 is a tetracyclic polypeptide of 19 amino acids that recognizes strictly the structure of phosphatidylethanolamine (PE) and forms a tight equimolar complex with PE on biological membranes. Using the cyclic peptide coupled with fluorescence-labeled streptavidin, we have analyzed the cell surface localization of PE in dividing Chinese hamster ovary cells. We found that PE was exposed on the cell surface specifically at the cleavage furrow during the late telophase of cytokinesis. PE was exposed on the cell surface only during the late telophase and no alteration in the distribution of the plasma membrane-bound cyclic peptide was observed during the cytokinesis, suggesting that the surface exposure of PE reflects the enhanced scrambling of PE at the cleavage furrow. Furthermore, cell surface immobilization of PE induced by adding the cyclic peptide coupled with streptavidin to prometaphase cells effectively blocked the cytokinesis at late telophase. The peptide-streptavidin complex treatment had no effect on furrowing, rearrangement of microtubules, and nuclear reconstitution, but specifically inhibited both actin filament disassembly at the cleavage furrow and subsequent membrane fusion. These results suggest that the redistribution of the plasma membrane phospholipids is a crucial step for cytokinesis and the cell surface PE may play a pivotal role in mediating a coordinate movement between the contractile ring and plasma membrane to achieve successful cell division.

Authors+Show Affiliations

Department of Inflammation Research, Tokyo Metropolitan Institute of Medical Science (RINSHOKEN), Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8917511

Citation

Emoto, K, et al. "Redistribution of Phosphatidylethanolamine at the Cleavage Furrow of Dividing Cells During Cytokinesis." Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 23, 1996, pp. 12867-72.
Emoto K, Kobayashi T, Yamaji A, et al. Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis. Proc Natl Acad Sci USA. 1996;93(23):12867-72.
Emoto, K., Kobayashi, T., Yamaji, A., Aizawa, H., Yahara, I., Inoue, K., & Umeda, M. (1996). Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis. Proceedings of the National Academy of Sciences of the United States of America, 93(23), 12867-72.
Emoto K, et al. Redistribution of Phosphatidylethanolamine at the Cleavage Furrow of Dividing Cells During Cytokinesis. Proc Natl Acad Sci USA. 1996 Nov 12;93(23):12867-72. PubMed PMID: 8917511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis. AU - Emoto,K, AU - Kobayashi,T, AU - Yamaji,A, AU - Aizawa,H, AU - Yahara,I, AU - Inoue,K, AU - Umeda,M, PY - 1996/11/12/pubmed PY - 1996/11/12/medline PY - 1996/11/12/entrez SP - 12867 EP - 72 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 93 IS - 23 N2 - Ro09-0198 is a tetracyclic polypeptide of 19 amino acids that recognizes strictly the structure of phosphatidylethanolamine (PE) and forms a tight equimolar complex with PE on biological membranes. Using the cyclic peptide coupled with fluorescence-labeled streptavidin, we have analyzed the cell surface localization of PE in dividing Chinese hamster ovary cells. We found that PE was exposed on the cell surface specifically at the cleavage furrow during the late telophase of cytokinesis. PE was exposed on the cell surface only during the late telophase and no alteration in the distribution of the plasma membrane-bound cyclic peptide was observed during the cytokinesis, suggesting that the surface exposure of PE reflects the enhanced scrambling of PE at the cleavage furrow. Furthermore, cell surface immobilization of PE induced by adding the cyclic peptide coupled with streptavidin to prometaphase cells effectively blocked the cytokinesis at late telophase. The peptide-streptavidin complex treatment had no effect on furrowing, rearrangement of microtubules, and nuclear reconstitution, but specifically inhibited both actin filament disassembly at the cleavage furrow and subsequent membrane fusion. These results suggest that the redistribution of the plasma membrane phospholipids is a crucial step for cytokinesis and the cell surface PE may play a pivotal role in mediating a coordinate movement between the contractile ring and plasma membrane to achieve successful cell division. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/8917511/Redistribution_of_phosphatidylethanolamine_at_the_cleavage_furrow_of_dividing_cells_during_cytokinesis_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=8917511 DB - PRIME DP - Unbound Medicine ER -