Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression.Gene. 1996 Oct 24; 177(1-2):267-8.GENE
An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Pol into the attB site of phage lambda in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage lambda.