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Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene.
Clin Infect Dis. 1996 Nov; 23(5):1002-6.CI

Abstract

Microsporidia are emerging as opportunistic pathogens in patients with AIDS. Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis have been implicated in enteric infections in AIDS patients with chronic diarrhea, a wasting syndrome, and malabsorption. We used the polymerase chain reaction (PCR) and primers that amplify the conserved regions of the small-subunit rRNA (SSU-rRNA) gene of E. bieneusi and E. intestinalis in tissue specimens from HIV-infected patients with and without diarrhea to examine the association between microsporidia and diarrhea in patients with AIDS. Tissue specimens were obtained from 68 patients with AIDS and diarrhea (mean CD4 lymphocyte count, 21/mm3) and 43 AIDS patients without diarrhea (mean CD4 lymphocyte count, 60/mm3). By means of PCR with use of the SSU-rRNA primers specific for E. bieneusi and E. intestinalis, we found that 44% of patients with diarrhea were infected with microsporidia, whereas only 2.3% of the patients without diarrhea were infected with microsporidia (P < .001). There was a clear association between the presence of microsporidia and diarrhea. In addition, the SSU-rRNA primers proved to be sensitive and specific when used in this clinical setting.

Authors+Show Affiliations

Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8922793

Citation

Coyle, C M., et al. "Prevalence of Microsporidiosis Due to Enterocytozoon Bieneusi and Encephalitozoon (Septata) Intestinalis Among Patients With AIDS-related Diarrhea: Determination By Polymerase Chain Reaction to the Microsporidian Small-subunit rRNA Gene." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, vol. 23, no. 5, 1996, pp. 1002-6.
Coyle CM, Wittner M, Kotler DP, et al. Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene. Clin Infect Dis. 1996;23(5):1002-6.
Coyle, C. M., Wittner, M., Kotler, D. P., Noyer, C., Orenstein, J. M., Tanowitz, H. B., & Weiss, L. M. (1996). Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene. Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, 23(5), 1002-6.
Coyle CM, et al. Prevalence of Microsporidiosis Due to Enterocytozoon Bieneusi and Encephalitozoon (Septata) Intestinalis Among Patients With AIDS-related Diarrhea: Determination By Polymerase Chain Reaction to the Microsporidian Small-subunit rRNA Gene. Clin Infect Dis. 1996;23(5):1002-6. PubMed PMID: 8922793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene. AU - Coyle,C M, AU - Wittner,M, AU - Kotler,D P, AU - Noyer,C, AU - Orenstein,J M, AU - Tanowitz,H B, AU - Weiss,L M, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 1002 EP - 6 JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America JO - Clin Infect Dis VL - 23 IS - 5 N2 - Microsporidia are emerging as opportunistic pathogens in patients with AIDS. Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis have been implicated in enteric infections in AIDS patients with chronic diarrhea, a wasting syndrome, and malabsorption. We used the polymerase chain reaction (PCR) and primers that amplify the conserved regions of the small-subunit rRNA (SSU-rRNA) gene of E. bieneusi and E. intestinalis in tissue specimens from HIV-infected patients with and without diarrhea to examine the association between microsporidia and diarrhea in patients with AIDS. Tissue specimens were obtained from 68 patients with AIDS and diarrhea (mean CD4 lymphocyte count, 21/mm3) and 43 AIDS patients without diarrhea (mean CD4 lymphocyte count, 60/mm3). By means of PCR with use of the SSU-rRNA primers specific for E. bieneusi and E. intestinalis, we found that 44% of patients with diarrhea were infected with microsporidia, whereas only 2.3% of the patients without diarrhea were infected with microsporidia (P < .001). There was a clear association between the presence of microsporidia and diarrhea. In addition, the SSU-rRNA primers proved to be sensitive and specific when used in this clinical setting. SN - 1058-4838 UR - https://www.unboundmedicine.com/medline/citation/8922793/Prevalence_of_microsporidiosis_due_to_Enterocytozoon_bieneusi_and_Encephalitozoon__Septata__intestinalis_among_patients_with_AIDS_related_diarrhea:_determination_by_polymerase_chain_reaction_to_the_microsporidian_small_subunit_rRNA_gene_ L2 - https://academic.oup.com/cid/article-lookup/doi/10.1093/clinids/23.5.1002 DB - PRIME DP - Unbound Medicine ER -