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Antisense oligodeoxynucleotide to a delta-opioid receptor messenger RNA selectively blocks the antinociception induced by intracerebroventricularly administered delta-, but not mu-, epsilon- or kappa-opioid receptor agonists in the mouse.
Neuroscience. 1996 Nov; 75(2):445-52.N

Abstract

An antisense oligodeoxynucleotide to delta-opioid receptor messenger RNA was utilized to block the expression of mouse delta-opioid receptors for antinociception. The antinociception was measured by the tail-flick test in male ICR mice. Pretreatment with delta-antisense oligodeoxynucleotide (163 pmol) given intracerebroventricularly twice a day for one to four days produced a time-dependent inhibition of the tail-flick response induced by intracerebroventricularly administered (D-Ala2)deltorphin II (12.8 nmol). The (D-Ala2)deltorphin II-induced antinociception was significantly attenuated after three to four days of the delta-antisense oligodeoxynucleotide treatment, remained attenuated for two days and gradually recovered to the control level in four to 10 days after cessation of the pretreatment with delta-antisense oligodeoxynucleotide. Pretreatment with delta-antisense oligodeoxynucleotide (163 pmol) twice a day for four days markedly attenuated the antinociception induced by intracerebroventricularly administered (D-Ala2)deltorphin II and, to a lesser extent, by D-Pen2-D-Pen5-enkephalin and morphine, but not by (D-Ala2-MePhe4-Gly(ol)5)enkephalin, beta-endorphin or U50,488H. Mismatched oligodeoxynucleotide (163 pmol) was ineffective against the antinociception induced by these opioids. Our results provide the evidence that the cloned delta-opioid receptor is related to the pharmacologically classified delta 2-opioid receptor, and the antinociception induced by (D-Ala2)deltorphin II and, at least in part, by D-Pen2-D-Pen5-enkephalin and morphine given intracerebroventricularly is mediated by the stimulation of delta 2-opioid receptors. However, delta 2-opioid receptors are not involved in the antinociception induced by beta-endorphin, (D-Ala2-MePhe4-Gly(ol)5)enkephalin or U50,488H given intracerebroventricularly.

Authors+Show Affiliations

Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8931008

Citation

Wang, H Q., et al. "Antisense Oligodeoxynucleotide to a Delta-opioid Receptor Messenger RNA Selectively Blocks the Antinociception Induced By Intracerebroventricularly Administered Delta-, but Not Mu-, Epsilon- or Kappa-opioid Receptor Agonists in the Mouse." Neuroscience, vol. 75, no. 2, 1996, pp. 445-52.
Wang HQ, Kampine JP, Tseng LF. Antisense oligodeoxynucleotide to a delta-opioid receptor messenger RNA selectively blocks the antinociception induced by intracerebroventricularly administered delta-, but not mu-, epsilon- or kappa-opioid receptor agonists in the mouse. Neuroscience. 1996;75(2):445-52.
Wang, H. Q., Kampine, J. P., & Tseng, L. F. (1996). Antisense oligodeoxynucleotide to a delta-opioid receptor messenger RNA selectively blocks the antinociception induced by intracerebroventricularly administered delta-, but not mu-, epsilon- or kappa-opioid receptor agonists in the mouse. Neuroscience, 75(2), 445-52.
Wang HQ, Kampine JP, Tseng LF. Antisense Oligodeoxynucleotide to a Delta-opioid Receptor Messenger RNA Selectively Blocks the Antinociception Induced By Intracerebroventricularly Administered Delta-, but Not Mu-, Epsilon- or Kappa-opioid Receptor Agonists in the Mouse. Neuroscience. 1996;75(2):445-52. PubMed PMID: 8931008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antisense oligodeoxynucleotide to a delta-opioid receptor messenger RNA selectively blocks the antinociception induced by intracerebroventricularly administered delta-, but not mu-, epsilon- or kappa-opioid receptor agonists in the mouse. AU - Wang,H Q, AU - Kampine,J P, AU - Tseng,L F, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 445 EP - 52 JF - Neuroscience JO - Neuroscience VL - 75 IS - 2 N2 - An antisense oligodeoxynucleotide to delta-opioid receptor messenger RNA was utilized to block the expression of mouse delta-opioid receptors for antinociception. The antinociception was measured by the tail-flick test in male ICR mice. Pretreatment with delta-antisense oligodeoxynucleotide (163 pmol) given intracerebroventricularly twice a day for one to four days produced a time-dependent inhibition of the tail-flick response induced by intracerebroventricularly administered (D-Ala2)deltorphin II (12.8 nmol). The (D-Ala2)deltorphin II-induced antinociception was significantly attenuated after three to four days of the delta-antisense oligodeoxynucleotide treatment, remained attenuated for two days and gradually recovered to the control level in four to 10 days after cessation of the pretreatment with delta-antisense oligodeoxynucleotide. Pretreatment with delta-antisense oligodeoxynucleotide (163 pmol) twice a day for four days markedly attenuated the antinociception induced by intracerebroventricularly administered (D-Ala2)deltorphin II and, to a lesser extent, by D-Pen2-D-Pen5-enkephalin and morphine, but not by (D-Ala2-MePhe4-Gly(ol)5)enkephalin, beta-endorphin or U50,488H. Mismatched oligodeoxynucleotide (163 pmol) was ineffective against the antinociception induced by these opioids. Our results provide the evidence that the cloned delta-opioid receptor is related to the pharmacologically classified delta 2-opioid receptor, and the antinociception induced by (D-Ala2)deltorphin II and, at least in part, by D-Pen2-D-Pen5-enkephalin and morphine given intracerebroventricularly is mediated by the stimulation of delta 2-opioid receptors. However, delta 2-opioid receptors are not involved in the antinociception induced by beta-endorphin, (D-Ala2-MePhe4-Gly(ol)5)enkephalin or U50,488H given intracerebroventricularly. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/8931008/Antisense_oligodeoxynucleotide_to_a_delta_opioid_receptor_messenger_RNA_selectively_blocks_the_antinociception_induced_by_intracerebroventricularly_administered_delta__but_not_mu__epsilon__or_kappa_opioid_receptor_agonists_in_the_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0306452296003090 DB - PRIME DP - Unbound Medicine ER -