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Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA.
FEMS Microbiol Lett 1996; 145(1):9-16FM

Abstract

Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isolates, and simultaneous confirmation of the species identity independent of plasmid content. The assay amplifies lef, cya, pag (pXO1) and cap (pXO2) genes, and a B. anthracis specific chromosomal marker, giving an easy-to-read profile. This system unambiguously identified virulent (pXO1+/2+) and avirulent (pXO1+/2-, pXO1-/2+ and pXO1-/2-) strains of B. anthracis and distinguished "anthrax-like' strains from other B. cereus group bacteria.

Authors+Show Affiliations

Laboratoire de Microbiologie Appliquée, Centre d'Etudes du Bouchet, Vert-Le-Petit, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8931320

Citation

Ramisse, V, et al. "Identification and Characterization of Bacillus Anthracis By Multiplex PCR Analysis of Sequences On Plasmids pXO1 and pXO2 and Chromosomal DNA." FEMS Microbiology Letters, vol. 145, no. 1, 1996, pp. 9-16.
Ramisse V, Patra G, Garrigue H, et al. Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. FEMS Microbiol Lett. 1996;145(1):9-16.
Ramisse, V., Patra, G., Garrigue, H., Guesdon, J. L., & Mock, M. (1996). Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. FEMS Microbiology Letters, 145(1), pp. 9-16.
Ramisse V, et al. Identification and Characterization of Bacillus Anthracis By Multiplex PCR Analysis of Sequences On Plasmids pXO1 and pXO2 and Chromosomal DNA. FEMS Microbiol Lett. 1996 Nov 15;145(1):9-16. PubMed PMID: 8931320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. AU - Ramisse,V, AU - Patra,G, AU - Garrigue,H, AU - Guesdon,J L, AU - Mock,M, PY - 1996/11/15/pubmed PY - 1996/11/15/medline PY - 1996/11/15/entrez SP - 9 EP - 16 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 145 IS - 1 N2 - Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isolates, and simultaneous confirmation of the species identity independent of plasmid content. The assay amplifies lef, cya, pag (pXO1) and cap (pXO2) genes, and a B. anthracis specific chromosomal marker, giving an easy-to-read profile. This system unambiguously identified virulent (pXO1+/2+) and avirulent (pXO1+/2-, pXO1-/2+ and pXO1-/2-) strains of B. anthracis and distinguished "anthrax-like' strains from other B. cereus group bacteria. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/8931320/Identification_and_characterization_of_Bacillus_anthracis_by_multiplex_PCR_analysis_of_sequences_on_plasmids_pXO1_and_pXO2_and_chromosomal_DNA_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.1996.tb08548.x DB - PRIME DP - Unbound Medicine ER -