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Localization of anthracycline accumulation in sensitive and resistant urothelial tumor cell lines.
Cancer Detect Prev. 1996; 20(6):625-33.CD

Abstract

Emergence of resistance to chemotherapy is a serious clinical problem. Our aim was to investigate anthracycline uptake in sensitive and resistant urothelial cancer cell lines and to manipulate uptake by resistant cells. Anthracyclines exhibit natural fluorescence; therefore, the techniques used for detection were high-resolution fluorescence image analysis and confocal microscopy. Different patterns of intracellular drug distribution were observed in the two cell lines. Sensitive cells showed predominantly nuclear drug localization, while in resistant cells there was nuclear and cytoplasmic fluorescence. Manipulation of drug uptake by resistant cells was achieved using an essential fatty acid, gammalinolenic acid, and the calcium channel blocker, verapamil. Both substances increased drug uptake in resistant cells, with verapamil producing patterns of intracellular drug distribution in resistant cells similar to those of sensitive cells.

Authors+Show Affiliations

Department of Surgery, University College, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8939349

Citation

Davies, C L., et al. "Localization of Anthracycline Accumulation in Sensitive and Resistant Urothelial Tumor Cell Lines." Cancer Detection and Prevention, vol. 20, no. 6, 1996, pp. 625-33.
Davies CL, Duffy PM, MacRobert AJ, et al. Localization of anthracycline accumulation in sensitive and resistant urothelial tumor cell lines. Cancer Detect Prev. 1996;20(6):625-33.
Davies, C. L., Duffy, P. M., MacRobert, A. J., Loizidou, M. C., Cooper, A. J., & Taylor, I. (1996). Localization of anthracycline accumulation in sensitive and resistant urothelial tumor cell lines. Cancer Detection and Prevention, 20(6), 625-33.
Davies CL, et al. Localization of Anthracycline Accumulation in Sensitive and Resistant Urothelial Tumor Cell Lines. Cancer Detect Prev. 1996;20(6):625-33. PubMed PMID: 8939349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of anthracycline accumulation in sensitive and resistant urothelial tumor cell lines. AU - Davies,C L, AU - Duffy,P M, AU - MacRobert,A J, AU - Loizidou,M C, AU - Cooper,A J, AU - Taylor,I, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 625 EP - 33 JF - Cancer detection and prevention JO - Cancer Detect Prev VL - 20 IS - 6 N2 - Emergence of resistance to chemotherapy is a serious clinical problem. Our aim was to investigate anthracycline uptake in sensitive and resistant urothelial cancer cell lines and to manipulate uptake by resistant cells. Anthracyclines exhibit natural fluorescence; therefore, the techniques used for detection were high-resolution fluorescence image analysis and confocal microscopy. Different patterns of intracellular drug distribution were observed in the two cell lines. Sensitive cells showed predominantly nuclear drug localization, while in resistant cells there was nuclear and cytoplasmic fluorescence. Manipulation of drug uptake by resistant cells was achieved using an essential fatty acid, gammalinolenic acid, and the calcium channel blocker, verapamil. Both substances increased drug uptake in resistant cells, with verapamil producing patterns of intracellular drug distribution in resistant cells similar to those of sensitive cells. SN - 0361-090X UR - https://www.unboundmedicine.com/medline/citation/8939349/Localization_of_anthracycline_accumulation_in_sensitive_and_resistant_urothelial_tumor_cell_lines_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=8939349.ui DB - PRIME DP - Unbound Medicine ER -