Tags

Type your tag names separated by a space and hit enter

Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR.
J Clin Microbiol. 1996 Dec; 34(12):3230-2.JC

Abstract

Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS.

Authors+Show Affiliations

Department of Parasitology, National Institute of Microbiology, Dr. Carlos G. Malbrán, Buenos Aires, Argentina. parasito@malbra.sld.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8940480

Citation

Velásquez, J N., et al. "Detection of the Microsporidian Parasite Enterocytozoon Bieneusi in Specimens From Patients With AIDS By PCR." Journal of Clinical Microbiology, vol. 34, no. 12, 1996, pp. 3230-2.
Velásquez JN, Carnevale S, Guarnera EA, et al. Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR. J Clin Microbiol. 1996;34(12):3230-2.
Velásquez, J. N., Carnevale, S., Guarnera, E. A., Labbé, J. H., Chertcoff, A., Cabrera, M. G., & Rodríguez, M. I. (1996). Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR. Journal of Clinical Microbiology, 34(12), 3230-2.
Velásquez JN, et al. Detection of the Microsporidian Parasite Enterocytozoon Bieneusi in Specimens From Patients With AIDS By PCR. J Clin Microbiol. 1996;34(12):3230-2. PubMed PMID: 8940480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR. AU - Velásquez,J N, AU - Carnevale,S, AU - Guarnera,E A, AU - Labbé,J H, AU - Chertcoff,A, AU - Cabrera,M G, AU - Rodríguez,M I, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 3230 EP - 2 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 34 IS - 12 N2 - Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/8940480/Detection_of_the_microsporidian_parasite_Enterocytozoon_bieneusi_in_specimens_from_patients_with_AIDS_by_PCR_ L2 - https://journals.asm.org/doi/10.1128/jcm.34.12.3230-3232.1996?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -