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The study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as matrices for DNA detection in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 1996; 10(3):383-8.RC

Abstract

The matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometric study of DNA detection using 2,3,4-trihydroxyacetophenone, 2,4,6-trihydroxyacetophenone, and their combination has been carried out systematically. The results show that a mixture of 2,4,6-trihydroxyacetophenone, 2,3,4-trihydroxyacetophenone and ammonium citrate with molar ratios of 2:1:1 serves as a good matrix for the detection of DNA, especially for samples containing a small quantity of DNA such as polymerase chain reaction product. The resolution and shot-to-shot reproducibility using this matrix are better than, and the MALDI sensitivity comparable to, that obtained when using 3-hydroxy picotinic acid (3-HPA), PA and ammonium citrate matrix (9:1:1). The mechanism of desorption/ionization using this matrix is discussed.

Authors+Show Affiliations

Zhu YF
Oak Ridge National Laboratory, TN 37831-6378, USA.
Chung CN
No affiliation info available
Taranenko NI
No affiliation info available
Allman SL
No affiliation info available
Martin SA
No affiliation info available
Haff L
No affiliation info available
Chen CH
No affiliation info available

MeSH

Base SequenceDNADNA PrimersMolecular Sequence DataPolymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8949485

Citation

Zhu, Y F., et al. "The Study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as Matrices for DNA Detection in Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 10, no. 3, 1996, pp. 383-8.
Zhu YF, Chung CN, Taranenko NI, et al. The study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as matrices for DNA detection in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 1996;10(3):383-8.
Zhu, Y. F., Chung, C. N., Taranenko, N. I., Allman, S. L., Martin, S. A., Haff, L., & Chen, C. H. (1996). The study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as matrices for DNA detection in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 10(3), 383-8.
Zhu YF, et al. The Study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as Matrices for DNA Detection in Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 1996;10(3):383-8. PubMed PMID: 8949485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The study of 2,3,4-trihydroxyacetophenone and 2,4,6-trihydroxyacetophenone as matrices for DNA detection in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Zhu,Y F, AU - Chung,C N, AU - Taranenko,N I, AU - Allman,S L, AU - Martin,S A, AU - Haff,L, AU - Chen,C H, PY - 1996/1/1/pubmed PY - 2000/6/20/medline PY - 1996/1/1/entrez SP - 383 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 10 IS - 3 N2 - The matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometric study of DNA detection using 2,3,4-trihydroxyacetophenone, 2,4,6-trihydroxyacetophenone, and their combination has been carried out systematically. The results show that a mixture of 2,4,6-trihydroxyacetophenone, 2,3,4-trihydroxyacetophenone and ammonium citrate with molar ratios of 2:1:1 serves as a good matrix for the detection of DNA, especially for samples containing a small quantity of DNA such as polymerase chain reaction product. The resolution and shot-to-shot reproducibility using this matrix are better than, and the MALDI sensitivity comparable to, that obtained when using 3-hydroxy picotinic acid (3-HPA), PA and ammonium citrate matrix (9:1:1). The mechanism of desorption/ionization using this matrix is discussed. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/8949485/The_study_of_234_trihydroxyacetophenone_and_246_trihydroxyacetophenone_as_matrices_for_DNA_detection_in_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/(SICI)1097-0231(199602)10:3<383::AID-RCM485>3.0.CO;2-W DB - PRIME DP - Unbound Medicine ER -
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