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Acceleration of somitic myogenesis in embryos of myogenin promoter-MRF4 transgenic mice.
Dev Dyn. 1996 Dec; 207(4):382-94.DD

Abstract

The four muscle regulatory factors (MRFs) of the MyoD family are expressed in distinct temporal and spatial patterns in developing somites. To examine MRF function and regulation in somites, we generated myogenin promoter-MRF4 transgenic mice in which MRF4 was expressed in rostral somites about a half day earlier than normal. We found that the transgene, which was expressed at about the same level as endogenous MRFs, did not noticeably alter developing or adult mice, whereas the rostral somites of transgenic embryos showed accelerated myocyte formation, as well as precocious expression of the endogenous MRF4 gene. In an individual transgenic somite, MRF4 was expressed in both presumptive myotomal (mesenchymal) and dermatomal (epithelial) cells. Transgenic dermatomal cells also contained myogenin, which is expressed early in myogenesis, but did not contain myosin, which is expressed late in myogenesis. In transgenic myotomal cells, in contrast, precocious expression of MRF4 accelerated late events in myogenesis, including myosin expression and striated myofibril formation. MRF function, therefore, appears to be differentially regulated in dermatomal and myotomal cells.

Authors+Show Affiliations

Block NE
Neuromuscular Laboratory, Massachusetts General Hospital, Charlestown 02129, USA.
Zhu Z
No affiliation info available
Kachinsky AM
No affiliation info available
Dominov JA
No affiliation info available
Miller JB
No affiliation info available

MeSH

AnimalsDNA-Binding ProteinsEpitheliumGene Expression Regulation, DevelopmentalImmunohistochemistryMiceMice, TransgenicMorphogenesisMuscle ProteinsMusclesMyoD ProteinMyogenic Regulatory Factor 5Myogenic Regulatory FactorsMyogeninPromoter Regions, GeneticRatsRhombencephalonSomitesTrans-Activators

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8950513

Citation

Block, N E., et al. "Acceleration of Somitic Myogenesis in Embryos of Myogenin promoter-MRF4 Transgenic Mice." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 207, no. 4, 1996, pp. 382-94.
Block NE, Zhu Z, Kachinsky AM, et al. Acceleration of somitic myogenesis in embryos of myogenin promoter-MRF4 transgenic mice. Dev Dyn. 1996;207(4):382-94.
Block, N. E., Zhu, Z., Kachinsky, A. M., Dominov, J. A., & Miller, J. B. (1996). Acceleration of somitic myogenesis in embryos of myogenin promoter-MRF4 transgenic mice. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 207(4), 382-94.
Block NE, et al. Acceleration of Somitic Myogenesis in Embryos of Myogenin promoter-MRF4 Transgenic Mice. Dev Dyn. 1996;207(4):382-94. PubMed PMID: 8950513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acceleration of somitic myogenesis in embryos of myogenin promoter-MRF4 transgenic mice. AU - Block,N E, AU - Zhu,Z, AU - Kachinsky,A M, AU - Dominov,J A, AU - Miller,J B, PY - 1996/12/1/pubmed PY - 2000/6/22/medline PY - 1996/12/1/entrez SP - 382 EP - 94 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 207 IS - 4 N2 - The four muscle regulatory factors (MRFs) of the MyoD family are expressed in distinct temporal and spatial patterns in developing somites. To examine MRF function and regulation in somites, we generated myogenin promoter-MRF4 transgenic mice in which MRF4 was expressed in rostral somites about a half day earlier than normal. We found that the transgene, which was expressed at about the same level as endogenous MRFs, did not noticeably alter developing or adult mice, whereas the rostral somites of transgenic embryos showed accelerated myocyte formation, as well as precocious expression of the endogenous MRF4 gene. In an individual transgenic somite, MRF4 was expressed in both presumptive myotomal (mesenchymal) and dermatomal (epithelial) cells. Transgenic dermatomal cells also contained myogenin, which is expressed early in myogenesis, but did not contain myosin, which is expressed late in myogenesis. In transgenic myotomal cells, in contrast, precocious expression of MRF4 accelerated late events in myogenesis, including myosin expression and striated myofibril formation. MRF function, therefore, appears to be differentially regulated in dermatomal and myotomal cells. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/8950513/Acceleration_of_somitic_myogenesis_in_embryos_of_myogenin_promoter_MRF4_transgenic_mice_ L2 - https://doi.org/10.1002/(SICI)1097-0177(199612)207:4<382::AID-AJA3>3.0.CO;2-D DB - PRIME DP - Unbound Medicine ER -