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Co-ordinate regulation of Salmonella typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression.
Mol Microbiol. 1996 Nov; 22(4):703-14.MM

Abstract

During infection of their hosts, salmonellae enter intestinal epithelial cells. It has been proposed that when Salmonella typhimurium is present in the intestinal lumen, several environmental and regulatory conditions modulate the expression of invasion factors required for bacterial entry into host cells. We report here that the expression of six different S. typhimurium invasion genes encoded on SPI1 (Salmonella pathogenicity island 1) is co-ordinately regulated by oxygen, osmolarity, pH, PhoPQ, and HilA. HilA is a transcriptional activator of the OmpR/ToxR family that is also encoded on SPI1. We have found that HilA plays a central role in the co-ordinated regulation of invasion genes by environmental and regulatory conditions. HilA can activate the expression of two invasion gene-lacZY fusions on reporter plasmids in Escherichia coll, suggesting that HilA acts directly at invasion-gene promoters in S. typhimurium. We have found that the regulation of invasion genes by oxygen, osmolarity, pH, and PhoPQ is indirect and is mediated by regulation of hilA expression by these environmental and regulatory factors. We hypothesize that the complex and co-ordinate regulation of Invasion genes by HilA is an important feature of salmonella pathogenesis and allows salmonellae to enter intestinal epithelial cells.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetios, Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8951817

Citation

Bajaj, V, et al. "Co-ordinate Regulation of Salmonella Typhimurium Invasion Genes By Environmental and Regulatory Factors Is Mediated By Control of hilA Expression." Molecular Microbiology, vol. 22, no. 4, 1996, pp. 703-14.
Bajaj V, Lucas RL, Hwang C, et al. Co-ordinate regulation of Salmonella typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression. Mol Microbiol. 1996;22(4):703-14.
Bajaj, V., Lucas, R. L., Hwang, C., & Lee, C. A. (1996). Co-ordinate regulation of Salmonella typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression. Molecular Microbiology, 22(4), 703-14.
Bajaj V, et al. Co-ordinate Regulation of Salmonella Typhimurium Invasion Genes By Environmental and Regulatory Factors Is Mediated By Control of hilA Expression. Mol Microbiol. 1996;22(4):703-14. PubMed PMID: 8951817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-ordinate regulation of Salmonella typhimurium invasion genes by environmental and regulatory factors is mediated by control of hilA expression. AU - Bajaj,V, AU - Lucas,R L, AU - Hwang,C, AU - Lee,C A, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 703 EP - 14 JF - Molecular microbiology JO - Mol Microbiol VL - 22 IS - 4 N2 - During infection of their hosts, salmonellae enter intestinal epithelial cells. It has been proposed that when Salmonella typhimurium is present in the intestinal lumen, several environmental and regulatory conditions modulate the expression of invasion factors required for bacterial entry into host cells. We report here that the expression of six different S. typhimurium invasion genes encoded on SPI1 (Salmonella pathogenicity island 1) is co-ordinately regulated by oxygen, osmolarity, pH, PhoPQ, and HilA. HilA is a transcriptional activator of the OmpR/ToxR family that is also encoded on SPI1. We have found that HilA plays a central role in the co-ordinated regulation of invasion genes by environmental and regulatory conditions. HilA can activate the expression of two invasion gene-lacZY fusions on reporter plasmids in Escherichia coll, suggesting that HilA acts directly at invasion-gene promoters in S. typhimurium. We have found that the regulation of invasion genes by oxygen, osmolarity, pH, and PhoPQ is indirect and is mediated by regulation of hilA expression by these environmental and regulatory factors. We hypothesize that the complex and co-ordinate regulation of Invasion genes by HilA is an important feature of salmonella pathogenesis and allows salmonellae to enter intestinal epithelial cells. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/8951817/Co_ordinate_regulation_of_Salmonella_typhimurium_invasion_genes_by_environmental_and_regulatory_factors_is_mediated_by_control_of_hilA_expression_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=1996&volume=22&issue=4&spage=703 DB - PRIME DP - Unbound Medicine ER -