Tags

Type your tag names separated by a space and hit enter

Androgen receptor CAG repeat lengths in prostate cancer: correlation with age of onset.
J Clin Endocrinol Metab. 1996 Dec; 81(12):4400-5.JC

Abstract

The androgen receptor (AR) is a structurally conserved member of the nuclear receptor superfamily. The amino-terminal domain is required for transcriptional activation and contains a region of polyglutamine encoded by CAG trinucleotide repeats. In humans, the number of CAG repeats is polymorphic; the average number is 22 in Caucasian males. Expansion of CAG repeats in the AR has clinical implications for human disease. As androgen influences prostate cancer growth, polymorphisms in CAG repeat length may affect the clinical course of patients with prostate cancer. To test for an association between clinical parameters of human prostate cancer and CAG repeat length, we analyzed normal lymphocyte DNA from 109 patients. The CAG region of the AR was amplified by the PCR. Reaction products were then amplified using end-labeled internal primers, cut at the internal PstI site and assayed on sequencing gels using a sequence ladder as a size standard. Sequence analysis of several samples validated this method for measurement of CAG repeat number. The median age of patients was 63 yr (range, 42-83), with 104 Caucasian, 2 African American, 1 Asian, and 2 other racial origin. The median repeat length was 25 for patients with stage A, 22 for patients with stage B, 22 for patients with stage C, and 23 for patients presenting with stage D disease. A significant correlation between CAG repeat length and age at onset was observed, whereas correlations with stage, level of prostate-specific antigen at diagnosis, and time to prostate-specific antigen relapse were not significant. Shorter CAG repeat lengths may be associated with the development of prostate cancer in men at a younger age. These data suggest that CAG repeat length can affect the risk of developing prostate cancer.

Authors+Show Affiliations

Center for Biomedical Research, Population Council, New York, New York 10021, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8954049

Citation

Hardy, D O., et al. "Androgen Receptor CAG Repeat Lengths in Prostate Cancer: Correlation With Age of Onset." The Journal of Clinical Endocrinology and Metabolism, vol. 81, no. 12, 1996, pp. 4400-5.
Hardy DO, Scher HI, Bogenreider T, et al. Androgen receptor CAG repeat lengths in prostate cancer: correlation with age of onset. J Clin Endocrinol Metab. 1996;81(12):4400-5.
Hardy, D. O., Scher, H. I., Bogenreider, T., Sabbatini, P., Zhang, Z. F., Nanus, D. M., & Catterall, J. F. (1996). Androgen receptor CAG repeat lengths in prostate cancer: correlation with age of onset. The Journal of Clinical Endocrinology and Metabolism, 81(12), 4400-5.
Hardy DO, et al. Androgen Receptor CAG Repeat Lengths in Prostate Cancer: Correlation With Age of Onset. J Clin Endocrinol Metab. 1996;81(12):4400-5. PubMed PMID: 8954049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen receptor CAG repeat lengths in prostate cancer: correlation with age of onset. AU - Hardy,D O, AU - Scher,H I, AU - Bogenreider,T, AU - Sabbatini,P, AU - Zhang,Z F, AU - Nanus,D M, AU - Catterall,J F, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 4400 EP - 5 JF - The Journal of clinical endocrinology and metabolism JO - J. Clin. Endocrinol. Metab. VL - 81 IS - 12 N2 - The androgen receptor (AR) is a structurally conserved member of the nuclear receptor superfamily. The amino-terminal domain is required for transcriptional activation and contains a region of polyglutamine encoded by CAG trinucleotide repeats. In humans, the number of CAG repeats is polymorphic; the average number is 22 in Caucasian males. Expansion of CAG repeats in the AR has clinical implications for human disease. As androgen influences prostate cancer growth, polymorphisms in CAG repeat length may affect the clinical course of patients with prostate cancer. To test for an association between clinical parameters of human prostate cancer and CAG repeat length, we analyzed normal lymphocyte DNA from 109 patients. The CAG region of the AR was amplified by the PCR. Reaction products were then amplified using end-labeled internal primers, cut at the internal PstI site and assayed on sequencing gels using a sequence ladder as a size standard. Sequence analysis of several samples validated this method for measurement of CAG repeat number. The median age of patients was 63 yr (range, 42-83), with 104 Caucasian, 2 African American, 1 Asian, and 2 other racial origin. The median repeat length was 25 for patients with stage A, 22 for patients with stage B, 22 for patients with stage C, and 23 for patients presenting with stage D disease. A significant correlation between CAG repeat length and age at onset was observed, whereas correlations with stage, level of prostate-specific antigen at diagnosis, and time to prostate-specific antigen relapse were not significant. Shorter CAG repeat lengths may be associated with the development of prostate cancer in men at a younger age. These data suggest that CAG repeat length can affect the risk of developing prostate cancer. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/8954049/Androgen_receptor_CAG_repeat_lengths_in_prostate_cancer:_correlation_with_age_of_onset_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.81.12.8954049 DB - PRIME DP - Unbound Medicine ER -