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Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937.
J Bacteriol. 1996 Dec; 178(24):7187-96.JB

Abstract

The phytopathogenic enterobacterium Erwinia chrysanthemi 3937 produces five major and several secondary endo-pectate lyases encoded by the pel genes. Most of these genes are arranged in clusters on the bacterial chromosome. The genomic region surrounding the pelB-pelC cluster was supposed to be involved in the regulation of PelB and PelC synthesis. We demonstrated that the variation of pelB expression resulted from the titration of a regulatory protein by the gene adjacent to pelC. This gene was renamed pelZ since it encodes a protein of 420 amino acids with an endo-pectate lyase activity. Regulation of pelZ expression was investigated by using transcriptional fusions and a study of mRNA synthesis. Its transcription depends on different environmental conditions. It is induced in planta and in the presence of pectic catabolite products. This induction seems to be partially mediated by the KdgR protein but does not result from a direct interaction of KdgR with the pelZ 5' region. The transcription of pelZ leads to the synthesis of a monocistronic mRNA. However, the synthesis of a polycistronic mRNA from the pelC promoter, regulated by KdgR, is responsible for increased production of PelZ under inducing conditions. pelZ transcription is also controlled by pecT, which regulates some other pel genes, but it is independent of the pecS regulatory locus. The pelZ gene appears to be widespread in different strains of E. chrysanthemi. Moreover, a gene homologous to pelZ exists in Erwinia carotovora subsp. atroseptica adjacent to the cluster containing the pectate lyase-encoding genes pel1, pel2, and pel3. This conservation could reflect a significant role of PelZ in the pectinolytic system of Erwiniae. We showed pelZ is not a predominant virulence factor of E. chrysanthemi but is involved in host specificity.

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Authors+Show Affiliations

Pissavin C
Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, UMR 5577, INSA, Villeurbanne, France.
Robert-Baudouy J
No affiliation info available
Hugouvieux-Cotte-Pattat N
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceCarbohydrate SequenceCloning, MolecularDNA, BacterialDickeya chrysanthemiEscherichia coliGene Expression Regulation, BacterialGlucoseIsoenzymesMolecular Sequence DataMultigene FamilyMutationNitrogenOsmolar ConcentrationPolysaccharide-LyasesSequence Homology, Nucleic AcidTranscription, GeneticVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8955401

Citation

Pissavin, C, et al. "Regulation of pelZ, a Gene of the pelB-pelC Cluster Encoding a New Pectate Lyase of Erwinia Chrysanthemi 3937." Journal of Bacteriology, vol. 178, no. 24, 1996, pp. 7187-96.
Pissavin C, Robert-Baudouy J, Hugouvieux-Cotte-Pattat N. Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937. J Bacteriol. 1996;178(24):7187-96.
Pissavin, C., Robert-Baudouy, J., & Hugouvieux-Cotte-Pattat, N. (1996). Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937. Journal of Bacteriology, 178(24), 7187-96.
Pissavin C, Robert-Baudouy J, Hugouvieux-Cotte-Pattat N. Regulation of pelZ, a Gene of the pelB-pelC Cluster Encoding a New Pectate Lyase of Erwinia Chrysanthemi 3937. J Bacteriol. 1996;178(24):7187-96. PubMed PMID: 8955401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937. AU - Pissavin,C, AU - Robert-Baudouy,J, AU - Hugouvieux-Cotte-Pattat,N, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 7187 EP - 96 JF - Journal of bacteriology JO - J Bacteriol VL - 178 IS - 24 N2 - The phytopathogenic enterobacterium Erwinia chrysanthemi 3937 produces five major and several secondary endo-pectate lyases encoded by the pel genes. Most of these genes are arranged in clusters on the bacterial chromosome. The genomic region surrounding the pelB-pelC cluster was supposed to be involved in the regulation of PelB and PelC synthesis. We demonstrated that the variation of pelB expression resulted from the titration of a regulatory protein by the gene adjacent to pelC. This gene was renamed pelZ since it encodes a protein of 420 amino acids with an endo-pectate lyase activity. Regulation of pelZ expression was investigated by using transcriptional fusions and a study of mRNA synthesis. Its transcription depends on different environmental conditions. It is induced in planta and in the presence of pectic catabolite products. This induction seems to be partially mediated by the KdgR protein but does not result from a direct interaction of KdgR with the pelZ 5' region. The transcription of pelZ leads to the synthesis of a monocistronic mRNA. However, the synthesis of a polycistronic mRNA from the pelC promoter, regulated by KdgR, is responsible for increased production of PelZ under inducing conditions. pelZ transcription is also controlled by pecT, which regulates some other pel genes, but it is independent of the pecS regulatory locus. The pelZ gene appears to be widespread in different strains of E. chrysanthemi. Moreover, a gene homologous to pelZ exists in Erwinia carotovora subsp. atroseptica adjacent to the cluster containing the pectate lyase-encoding genes pel1, pel2, and pel3. This conservation could reflect a significant role of PelZ in the pectinolytic system of Erwiniae. We showed pelZ is not a predominant virulence factor of E. chrysanthemi but is involved in host specificity. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8955401/Regulation_of_pelZ_a_gene_of_the_pelB_pelC_cluster_encoding_a_new_pectate_lyase_of_Erwinia_chrysanthemi_3937_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8955401 DB - PRIME DP - Unbound Medicine ER -