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Characterization of the expression and gene promoter of CD22 in murine B cells.
Eur J Immunol. 1996 Dec; 26(12):3170-8.EJ

Abstract

CD22 is a B cell-restricted surface molecule which may play an important role in interactions between B cells and other cells and in regulating signals through the B cell receptor (BCR) complex. Here we have examined whether the mouse is a suitable in vivo model for studying CD22 functions. In primary and secondary lymphoid organs of adult mice CD22 is on all mature B cells, including resting IgM+IgD+ B cells, IgG+ HSA(lo) memory B cells, syndecan+ plasma cells and CD5+ B cells, but it is not on immature IgM+IgD- B cells. Biochemical analysis revealed that murine CD22 is associated with the IgM receptor in some, but not all, CD22+ B leukemic and lymphoma cell lines; as with human CD22, murine CD22 is rapidly phosphorylated on tyrosine after ligation of the BCR. In the CD22- murine pro-B cell line, FEMCL, CD22 expression was inducible by treatment with phorbol 12-myristate 13-acetate. A genomic fragment of the cd22b allele containing 1.3 kb 5' of exon 1 was sequenced in order to identify potential DNA regulatory elements in the CD22 promoter region. Consensus sequences for transcription factor binding sites including PU.1, AP-1, AP-2, C/EBP and SP-1 were present, but no classical TATA elements or initiator motifs were evident at relevant positions. The 1.3-kb promoter fragment 5' of exon 1 was sufficient for directing basal promoter activity in B and T cells. There was no significant sequence similarity between the murine and human cd22 gene promoters, although both contain repetitive elements and Sp-1 and AP1 binding sites. Thus, murine CD22 shares a number of features with human CD22 and the mouse provides a suitable model system for elucidating the function of CD22 in vivo.

Authors+Show Affiliations

Department of Microbiology, University of Washington, Seattle 98195-7242, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8977319

Citation

Andersson, K B., et al. "Characterization of the Expression and Gene Promoter of CD22 in Murine B Cells." European Journal of Immunology, vol. 26, no. 12, 1996, pp. 3170-8.
Andersson KB, Draves KE, Magaletti DM, et al. Characterization of the expression and gene promoter of CD22 in murine B cells. Eur J Immunol. 1996;26(12):3170-8.
Andersson, K. B., Draves, K. E., Magaletti, D. M., Fujioka, S., Holmes, K. L., Law, C. L., & Clark, E. A. (1996). Characterization of the expression and gene promoter of CD22 in murine B cells. European Journal of Immunology, 26(12), 3170-8.
Andersson KB, et al. Characterization of the Expression and Gene Promoter of CD22 in Murine B Cells. Eur J Immunol. 1996;26(12):3170-8. PubMed PMID: 8977319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the expression and gene promoter of CD22 in murine B cells. AU - Andersson,K B, AU - Draves,K E, AU - Magaletti,D M, AU - Fujioka,S, AU - Holmes,K L, AU - Law,C L, AU - Clark,E A, PY - 1996/12/1/pubmed PY - 1996/12/1/medline PY - 1996/12/1/entrez SP - 3170 EP - 8 JF - European journal of immunology JO - Eur J Immunol VL - 26 IS - 12 N2 - CD22 is a B cell-restricted surface molecule which may play an important role in interactions between B cells and other cells and in regulating signals through the B cell receptor (BCR) complex. Here we have examined whether the mouse is a suitable in vivo model for studying CD22 functions. In primary and secondary lymphoid organs of adult mice CD22 is on all mature B cells, including resting IgM+IgD+ B cells, IgG+ HSA(lo) memory B cells, syndecan+ plasma cells and CD5+ B cells, but it is not on immature IgM+IgD- B cells. Biochemical analysis revealed that murine CD22 is associated with the IgM receptor in some, but not all, CD22+ B leukemic and lymphoma cell lines; as with human CD22, murine CD22 is rapidly phosphorylated on tyrosine after ligation of the BCR. In the CD22- murine pro-B cell line, FEMCL, CD22 expression was inducible by treatment with phorbol 12-myristate 13-acetate. A genomic fragment of the cd22b allele containing 1.3 kb 5' of exon 1 was sequenced in order to identify potential DNA regulatory elements in the CD22 promoter region. Consensus sequences for transcription factor binding sites including PU.1, AP-1, AP-2, C/EBP and SP-1 were present, but no classical TATA elements or initiator motifs were evident at relevant positions. The 1.3-kb promoter fragment 5' of exon 1 was sufficient for directing basal promoter activity in B and T cells. There was no significant sequence similarity between the murine and human cd22 gene promoters, although both contain repetitive elements and Sp-1 and AP1 binding sites. Thus, murine CD22 shares a number of features with human CD22 and the mouse provides a suitable model system for elucidating the function of CD22 in vivo. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/8977319/Characterization_of_the_expression_and_gene_promoter_of_CD22_in_murine_B_cells_ L2 - https://doi.org/10.1002/eji.1830261250 DB - PRIME DP - Unbound Medicine ER -