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Stimulation of proliferation of an essential fatty acid-deficient fish cell line by C20 and C22 polyunsaturated fatty acids and effects on fatty acid composition.
Prostaglandins Leukot Essent Fatty Acids. 1996 Nov; 55(5):345-56.PL

Abstract

Recently we reported the development of a fish cell line, EPC-EFAD, derived from the carp (Cyprinus carpio) epithelial papilloma line, EPC, that could survive and proliferate in essential fatty acid-deficient (EFAD) medium. The EPC-EFAD cell line may be a useful model system in which to study the cellular biochemical effects of EFA deficiency and has advantages in studies of polyunsaturated fatty acid (PUFA) and eicosanoid metabolism in fish in that the complications introduced by culture in relatively n-6 PUFA-rich mammalian sera are removed. In the present study, the effects on cell proliferation rate of supplementing EPC-EFAD cells with various n-3 and n-6 PUFA were investigated to determine the possible role(s) of PUFA in cell growth and division. The selectivity of incorporation of specific PUFA into individual glycerophospholipid classes and the feasibility of reproducing in vivo fatty acid compositions in vitro were also investigated. Proliferation of the EPC-EFAD cell line was stimulated by arachidonic (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic (22:6 n-3) fatty acids but not by 18:2 n-6 or 18:3 n-3. The differential effects of PUFA on cellular proliferation may be related to the lack of significant delta 5 desaturase activity in the cells at 22 degrees C and may implicate a role for eicosanoids in the mechanism of stimulation of proliferation. PUFA supplementation increased the cytotoxic effects of longer term culture, an effect that was partly alleviated by inclusion of vitamin E in the culture medium. The cells could generally be supplemented with PUFA to produce cellular fatty acid compositions in vitro that were similar to in vivo compositions.

Authors+Show Affiliations

Department of Biological and Molecular Sciences, University of Stirling, Scotland, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8981632

Citation

Tocher, D R., et al. "Stimulation of Proliferation of an Essential Fatty Acid-deficient Fish Cell Line By C20 and C22 Polyunsaturated Fatty Acids and Effects On Fatty Acid Composition." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 55, no. 5, 1996, pp. 345-56.
Tocher DR, Dick JR, Sargent JR. Stimulation of proliferation of an essential fatty acid-deficient fish cell line by C20 and C22 polyunsaturated fatty acids and effects on fatty acid composition. Prostaglandins Leukot Essent Fatty Acids. 1996;55(5):345-56.
Tocher, D. R., Dick, J. R., & Sargent, J. R. (1996). Stimulation of proliferation of an essential fatty acid-deficient fish cell line by C20 and C22 polyunsaturated fatty acids and effects on fatty acid composition. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 55(5), 345-56.
Tocher DR, Dick JR, Sargent JR. Stimulation of Proliferation of an Essential Fatty Acid-deficient Fish Cell Line By C20 and C22 Polyunsaturated Fatty Acids and Effects On Fatty Acid Composition. Prostaglandins Leukot Essent Fatty Acids. 1996;55(5):345-56. PubMed PMID: 8981632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stimulation of proliferation of an essential fatty acid-deficient fish cell line by C20 and C22 polyunsaturated fatty acids and effects on fatty acid composition. AU - Tocher,D R, AU - Dick,J R, AU - Sargent,J R, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 345 EP - 56 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 55 IS - 5 N2 - Recently we reported the development of a fish cell line, EPC-EFAD, derived from the carp (Cyprinus carpio) epithelial papilloma line, EPC, that could survive and proliferate in essential fatty acid-deficient (EFAD) medium. The EPC-EFAD cell line may be a useful model system in which to study the cellular biochemical effects of EFA deficiency and has advantages in studies of polyunsaturated fatty acid (PUFA) and eicosanoid metabolism in fish in that the complications introduced by culture in relatively n-6 PUFA-rich mammalian sera are removed. In the present study, the effects on cell proliferation rate of supplementing EPC-EFAD cells with various n-3 and n-6 PUFA were investigated to determine the possible role(s) of PUFA in cell growth and division. The selectivity of incorporation of specific PUFA into individual glycerophospholipid classes and the feasibility of reproducing in vivo fatty acid compositions in vitro were also investigated. Proliferation of the EPC-EFAD cell line was stimulated by arachidonic (20:4 n-6), eicosapentaenoic (20:5 n-3) and docosahexaenoic (22:6 n-3) fatty acids but not by 18:2 n-6 or 18:3 n-3. The differential effects of PUFA on cellular proliferation may be related to the lack of significant delta 5 desaturase activity in the cells at 22 degrees C and may implicate a role for eicosanoids in the mechanism of stimulation of proliferation. PUFA supplementation increased the cytotoxic effects of longer term culture, an effect that was partly alleviated by inclusion of vitamin E in the culture medium. The cells could generally be supplemented with PUFA to produce cellular fatty acid compositions in vitro that were similar to in vivo compositions. SN - 0952-3278 UR - https://www.unboundmedicine.com/medline/citation/8981632/Stimulation_of_proliferation_of_an_essential_fatty_acid_deficient_fish_cell_line_by_C20_and_C22_polyunsaturated_fatty_acids_and_effects_on_fatty_acid_composition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(96)90041-1 DB - PRIME DP - Unbound Medicine ER -