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Diagnosis of measles with an IgM capture EIA: the optimal timing of specimen collection after rash onset.
J Infect Dis. 1997 Jan; 175(1):195-9.JI

Abstract

The optimal timing for collection of a single serum specimen to diagnose measles by using a monoclonal antibody-capture EIA was evaluated. Results of testing paired serum samples from 166 measles cases with at least 1 IgM-positive specimen were analyzed. Among persons whose second samples were IgM-positive, the seropositivity rate for first samples was 77% when collected within 72 h and 100% when collected 4-11 days after rash onset. Among unvaccinated persons whose first samples were IgM-positive, the rate for IgM positivity of second specimens declined from 100% at 4 days to 94% at 4 weeks after rash onset, then declined further to 63% at 5 weeks. Some previously vaccinated persons became IgM-negative during the third week after rash onset. In general, a single serum specimen collected between 72 h and 4 weeks after rash onset can be used to diagnose most cases of measles with an IgM capture EIA.

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Authors+Show Affiliations

Helfand RF
Department of Pediatrics, Emory University, Atlanta, Georgia, USA.
Heath JL
No affiliation info available
Anderson LJ
No affiliation info available
Maes EF
No affiliation info available
Guris D
No affiliation info available
Bellini WJ
No affiliation info available

MeSH

AdolescentAdultAntibodies, ViralBlood Specimen CollectionChildChild, PreschoolHumansImmunoenzyme TechniquesImmunoglobulin MInfantMeaslesMeasles VaccineMeasles virusMiddle AgedTime FactorsVaccination

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8985220

Citation

Helfand, R F., et al. "Diagnosis of Measles With an IgM Capture EIA: the Optimal Timing of Specimen Collection After Rash Onset." The Journal of Infectious Diseases, vol. 175, no. 1, 1997, pp. 195-9.
Helfand RF, Heath JL, Anderson LJ, et al. Diagnosis of measles with an IgM capture EIA: the optimal timing of specimen collection after rash onset. J Infect Dis. 1997;175(1):195-9.
Helfand, R. F., Heath, J. L., Anderson, L. J., Maes, E. F., Guris, D., & Bellini, W. J. (1997). Diagnosis of measles with an IgM capture EIA: the optimal timing of specimen collection after rash onset. The Journal of Infectious Diseases, 175(1), 195-9.
Helfand RF, et al. Diagnosis of Measles With an IgM Capture EIA: the Optimal Timing of Specimen Collection After Rash Onset. J Infect Dis. 1997;175(1):195-9. PubMed PMID: 8985220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of measles with an IgM capture EIA: the optimal timing of specimen collection after rash onset. AU - Helfand,R F, AU - Heath,J L, AU - Anderson,L J, AU - Maes,E F, AU - Guris,D, AU - Bellini,W J, PY - 1997/1/1/pubmed PY - 1997/1/1/medline PY - 1997/1/1/entrez SP - 195 EP - 9 JF - The Journal of infectious diseases JO - J Infect Dis VL - 175 IS - 1 N2 - The optimal timing for collection of a single serum specimen to diagnose measles by using a monoclonal antibody-capture EIA was evaluated. Results of testing paired serum samples from 166 measles cases with at least 1 IgM-positive specimen were analyzed. Among persons whose second samples were IgM-positive, the seropositivity rate for first samples was 77% when collected within 72 h and 100% when collected 4-11 days after rash onset. Among unvaccinated persons whose first samples were IgM-positive, the rate for IgM positivity of second specimens declined from 100% at 4 days to 94% at 4 weeks after rash onset, then declined further to 63% at 5 weeks. Some previously vaccinated persons became IgM-negative during the third week after rash onset. In general, a single serum specimen collected between 72 h and 4 weeks after rash onset can be used to diagnose most cases of measles with an IgM capture EIA. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/8985220/Diagnosis_of_measles_with_an_IgM_capture_EIA:_the_optimal_timing_of_specimen_collection_after_rash_onset_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/175.1.195 DB - PRIME DP - Unbound Medicine ER -