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Costimulation of CD28- T lymphocytes by 4-1BB ligand.
J Immunol. 1997 Jan 15; 158(2):551-9.JI

Abstract

The interaction of the T cell surface protein CD28 with its ligand, B7-1 or B7-2, provides a critical costimulatory signal for T cell activation. T cells from CD28- mice are deficient in a variety of responses, including those to lectins and allogeneic spleen cells. However, some immune responses do occur in CD28- mice, suggesting the existence of alternate costimulatory pathways. In this work, we show that T cells purified from CD28- mice respond to B lymphomas expressing 4-1BB ligand (4-1BBL), a member of the TNF gene family. This response is inhibited by a soluble form of 4-1BB, the T cell surface receptor for 4-1BBL. Thus, 4-1BBL/4-1BB interaction provides costimulatory signals to T cells independent of signaling through the CD28 receptor. We find that 4-1BBL is inducible on splenic B cells by CD40 ligand/CD40 interaction or by culturing of splenic dendritic cells, treatments that also induce B7 family molecules. CD28- T cells fail to respond in an MLR to resting allogeneic spleen cells. However, treatment of spleen cells with CD40 ligand renders them competent in activation of CD28- T cells. In contrast to results using B lymphomas as APC, soluble 4-1BB fails to inhibit the T cell response to activated spleen cells. This failure of soluble 4-1BB to block an MLR between CD28+ or CD28- T cells and allogeneic spleen cells is in contrast to a previous report with CD28+ cells.

Authors+Show Affiliations

Department of Immunology, University of Toronto, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8992967

Citation

DeBenedette, M A., et al. "Costimulation of CD28- T Lymphocytes By 4-1BB Ligand." Journal of Immunology (Baltimore, Md. : 1950), vol. 158, no. 2, 1997, pp. 551-9.
DeBenedette MA, Shahinian A, Mak TW, et al. Costimulation of CD28- T lymphocytes by 4-1BB ligand. J Immunol. 1997;158(2):551-9.
DeBenedette, M. A., Shahinian, A., Mak, T. W., & Watts, T. H. (1997). Costimulation of CD28- T lymphocytes by 4-1BB ligand. Journal of Immunology (Baltimore, Md. : 1950), 158(2), 551-9.
DeBenedette MA, et al. Costimulation of CD28- T Lymphocytes By 4-1BB Ligand. J Immunol. 1997 Jan 15;158(2):551-9. PubMed PMID: 8992967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Costimulation of CD28- T lymphocytes by 4-1BB ligand. AU - DeBenedette,M A, AU - Shahinian,A, AU - Mak,T W, AU - Watts,T H, PY - 1997/1/15/pubmed PY - 1997/1/15/medline PY - 1997/1/15/entrez SP - 551 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 158 IS - 2 N2 - The interaction of the T cell surface protein CD28 with its ligand, B7-1 or B7-2, provides a critical costimulatory signal for T cell activation. T cells from CD28- mice are deficient in a variety of responses, including those to lectins and allogeneic spleen cells. However, some immune responses do occur in CD28- mice, suggesting the existence of alternate costimulatory pathways. In this work, we show that T cells purified from CD28- mice respond to B lymphomas expressing 4-1BB ligand (4-1BBL), a member of the TNF gene family. This response is inhibited by a soluble form of 4-1BB, the T cell surface receptor for 4-1BBL. Thus, 4-1BBL/4-1BB interaction provides costimulatory signals to T cells independent of signaling through the CD28 receptor. We find that 4-1BBL is inducible on splenic B cells by CD40 ligand/CD40 interaction or by culturing of splenic dendritic cells, treatments that also induce B7 family molecules. CD28- T cells fail to respond in an MLR to resting allogeneic spleen cells. However, treatment of spleen cells with CD40 ligand renders them competent in activation of CD28- T cells. In contrast to results using B lymphomas as APC, soluble 4-1BB fails to inhibit the T cell response to activated spleen cells. This failure of soluble 4-1BB to block an MLR between CD28+ or CD28- T cells and allogeneic spleen cells is in contrast to a previous report with CD28+ cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8992967/Costimulation_of_CD28__T_lymphocytes_by_4_1BB_ligand_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=8992967 DB - PRIME DP - Unbound Medicine ER -