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Non-retinal abnormalities associated with progressive retinal atrophy (PRA) in the miniature poodle.
Exp Eye Res. 1996 Nov; 63(5):527-33.EE

Abstract

Osmotic fragility was examined in red blood cells taken from miniature poodles with progressive retinal atrophy (PRA). In this study, the median osmotic fragility for erythrocytes (concentration of NaCl required for 50% haemolysis) was found to be significantly lower for clinically affected, compared to unaffected animals. These differences were maintained regardless of changes in erythrocyte incubation pH (7.0, 7.5). There was no difference in the slope parameter of erythrocyte osmotic fragility profiles for both sets of animals, indicating a similar variability of erythrocyte age. In groups of animals maintained with the same diet and fasted for 12 hr, and in groups of animals with no dietary constraints, total plasma cholesterol was significantly lower for affected, compared to unaffected animals. Red blood cell indices were also assessed; mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red blood cell count (RBC) and blood haemoglobin concentration (Hb) were normal, while erythrocyte packed cell volume (PCV) was significantly reduced for affected, compared to unaffected. Data from this study suggest that the abnormal erythrocyte osmotic fragility of affected animals is unlikely to be a consequence of a higher erythrocyte surface area/volume ratio, higher plasma cholesterol concentration, or greater proportion of new to old erythrocytes, and that a general membrane defect might be associated with prcd in the miniature poodle.

Authors+Show Affiliations

United Medical School, Guy's Hospital, Department of Ophthalmology, Rayne Institute, London, U.K.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8994356

Citation

Willmott, N J., and A A. Hussain. "Non-retinal Abnormalities Associated With Progressive Retinal Atrophy (PRA) in the Miniature Poodle." Experimental Eye Research, vol. 63, no. 5, 1996, pp. 527-33.
Willmott NJ, Hussain AA. Non-retinal abnormalities associated with progressive retinal atrophy (PRA) in the miniature poodle. Exp Eye Res. 1996;63(5):527-33.
Willmott, N. J., & Hussain, A. A. (1996). Non-retinal abnormalities associated with progressive retinal atrophy (PRA) in the miniature poodle. Experimental Eye Research, 63(5), 527-33.
Willmott NJ, Hussain AA. Non-retinal Abnormalities Associated With Progressive Retinal Atrophy (PRA) in the Miniature Poodle. Exp Eye Res. 1996;63(5):527-33. PubMed PMID: 8994356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-retinal abnormalities associated with progressive retinal atrophy (PRA) in the miniature poodle. AU - Willmott,N J, AU - Hussain,A A, PY - 1996/11/1/pubmed PY - 1996/11/1/medline PY - 1996/11/1/entrez SP - 527 EP - 33 JF - Experimental eye research JO - Exp Eye Res VL - 63 IS - 5 N2 - Osmotic fragility was examined in red blood cells taken from miniature poodles with progressive retinal atrophy (PRA). In this study, the median osmotic fragility for erythrocytes (concentration of NaCl required for 50% haemolysis) was found to be significantly lower for clinically affected, compared to unaffected animals. These differences were maintained regardless of changes in erythrocyte incubation pH (7.0, 7.5). There was no difference in the slope parameter of erythrocyte osmotic fragility profiles for both sets of animals, indicating a similar variability of erythrocyte age. In groups of animals maintained with the same diet and fasted for 12 hr, and in groups of animals with no dietary constraints, total plasma cholesterol was significantly lower for affected, compared to unaffected animals. Red blood cell indices were also assessed; mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red blood cell count (RBC) and blood haemoglobin concentration (Hb) were normal, while erythrocyte packed cell volume (PCV) was significantly reduced for affected, compared to unaffected. Data from this study suggest that the abnormal erythrocyte osmotic fragility of affected animals is unlikely to be a consequence of a higher erythrocyte surface area/volume ratio, higher plasma cholesterol concentration, or greater proportion of new to old erythrocytes, and that a general membrane defect might be associated with prcd in the miniature poodle. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/8994356/Non_retinal_abnormalities_associated_with_progressive_retinal_atrophy__PRA__in_the_miniature_poodle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(96)90143-8 DB - PRIME DP - Unbound Medicine ER -