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EAT-2 is a novel SH2 domain containing protein that is up regulated by Ewing's sarcoma EWS/FLI1 fusion gene.
Oncogene. 1996 Dec 19; 13(12):2649-58.O

Abstract

The EWS/FLI1 fusion protein is created by the translocation between chromosomes 11 and 22 that appears in most Ewing's sarcomas. This chimeric protein has been demonstrated to be an aberrant transcription factor. Genes up regulated by EWS/FLI1 but not by full-length FLI1 were identified by representational difference analysis (RDA). We have characterized a novel gene, EWS/FLI1 activated transcript 2 (EAT-2) that was cloned from a murine cDNA library using a differentially expressed RDA fragment. EAT-2 expression is seen within 4-8 h of EWS/FLI1 induction. Its expression correlates with transformation of NIH3T3 cells by chimeric proteins related to EWS/FLI1 but not by unrelated genes. EAT-2 is expressed in normal murine tissues and contains a unique but biochemically functional SH2 domain. An homologous sequence in the human genome has been identified and mapped to chromosome 1q22. Human EAT-2 transcripts were identified by reverse transcriptase-polymerase chain reaction (RT-PCR) in Ewing's sarcoma cell tumour cell lines. EAT-2's unique structure and correlation with transformation make it a candidate for playing a role in the transformation of NIH3T3 cells and the oncogenesis of Ewing's sarcoma.

Authors+Show Affiliations

Molecular Biology Institute, University of California, Los Angeles 90095, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9000139

Citation

Thompson, A D., et al. "EAT-2 Is a Novel SH2 Domain Containing Protein That Is Up Regulated By Ewing's Sarcoma EWS/FLI1 Fusion Gene." Oncogene, vol. 13, no. 12, 1996, pp. 2649-58.
Thompson AD, Braun BS, Arvand A, et al. EAT-2 is a novel SH2 domain containing protein that is up regulated by Ewing's sarcoma EWS/FLI1 fusion gene. Oncogene. 1996;13(12):2649-58.
Thompson, A. D., Braun, B. S., Arvand, A., Stewart, S. D., May, W. A., Chen, E., Korenberg, J., & Denny, C. (1996). EAT-2 is a novel SH2 domain containing protein that is up regulated by Ewing's sarcoma EWS/FLI1 fusion gene. Oncogene, 13(12), 2649-58.
Thompson AD, et al. EAT-2 Is a Novel SH2 Domain Containing Protein That Is Up Regulated By Ewing's Sarcoma EWS/FLI1 Fusion Gene. Oncogene. 1996 Dec 19;13(12):2649-58. PubMed PMID: 9000139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EAT-2 is a novel SH2 domain containing protein that is up regulated by Ewing's sarcoma EWS/FLI1 fusion gene. AU - Thompson,A D, AU - Braun,B S, AU - Arvand,A, AU - Stewart,S D, AU - May,W A, AU - Chen,E, AU - Korenberg,J, AU - Denny,C, PY - 1996/12/19/pubmed PY - 1996/12/19/medline PY - 1996/12/19/entrez SP - 2649 EP - 58 JF - Oncogene JO - Oncogene VL - 13 IS - 12 N2 - The EWS/FLI1 fusion protein is created by the translocation between chromosomes 11 and 22 that appears in most Ewing's sarcomas. This chimeric protein has been demonstrated to be an aberrant transcription factor. Genes up regulated by EWS/FLI1 but not by full-length FLI1 were identified by representational difference analysis (RDA). We have characterized a novel gene, EWS/FLI1 activated transcript 2 (EAT-2) that was cloned from a murine cDNA library using a differentially expressed RDA fragment. EAT-2 expression is seen within 4-8 h of EWS/FLI1 induction. Its expression correlates with transformation of NIH3T3 cells by chimeric proteins related to EWS/FLI1 but not by unrelated genes. EAT-2 is expressed in normal murine tissues and contains a unique but biochemically functional SH2 domain. An homologous sequence in the human genome has been identified and mapped to chromosome 1q22. Human EAT-2 transcripts were identified by reverse transcriptase-polymerase chain reaction (RT-PCR) in Ewing's sarcoma cell tumour cell lines. EAT-2's unique structure and correlation with transformation make it a candidate for playing a role in the transformation of NIH3T3 cells and the oncogenesis of Ewing's sarcoma. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/9000139/EAT_2_is_a_novel_SH2_domain_containing_protein_that_is_up_regulated_by_Ewing's_sarcoma_EWS/FLI1_fusion_gene_ L2 - http://amigo.geneontology.org/amigo/reference/PMID:9000139 DB - PRIME DP - Unbound Medicine ER -